来自受污染和控制地点的Opsius heydeni (昆虫,半,花科) 中线粒体DNA复制数变化的第一份报告
Giada Santa Calogero1, Marta Giuga1,2, Vera D'Urso1
1Department of Biological, Geological and Environmental Sciences, Section of Animal Biology "M. La Greca", University of Catania, Via Androne 81, 95124 Catania, Italy.
Animals : an open access journal from MDPI
|October 27, 2023
概括
环境污染物降低了Opsius heydeni中的线粒体DNA复制数 (mtDNAcn). 这种昆虫物种显示出作为评估环境质量和污染水平的生物指标的潜力.
科学领域:
- 环境科学 环境科学
- 分子生物学分子生物学
- 生态毒理学 生态毒理学
背景情况:
- 线粒体DNA (mtDNA) 容易受到环境压力因素的改变.
- 线粒体DNA拷贝数 (mtDNAcn) 变异作为环境污染物暴露的潜在生物标志物.
研究的目的:
- 调查Opsius heydeni中mtDNAcn变异作为对环境污染的反应.
- 确定Opsius heydeni作为环境质量评估的新生物指标物种.
主要方法:
- 在西西里的一个受污染地点 (Priolo Gargallo) 和一个控制地点 (Simeto河绿洲) 采集了Opsius heydeni样本.
- 用于物种识别和数据库贡献的COI基因的测序.
- 使用实时PCR准COI和18S基因的相对mtDNAcn的量化.
主要成果:
- 与对照地点相比,在受污染地点的Opsius heydeni标本中观察到mtDNAcn的显著下降.
- 为Opsius heydeni生成并提供了新的COI基因序列.
结论:
- 环境污染物可以诱导Opsius heydeni.的线粒体基因组的改变.
- 奥普西乌斯海德尼 (Opsius heydeni) 显示出作为一种敏感的生物指标物种,用于监测环境质量和检测污染影响的潜力.
相关概念视频
02:26Comparing Copy Number Variations and SNPs
17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K
02:59Animal Mitochondrial Genetics
7.6K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.6K