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识别Streptococcus pyogenes与宿主相互作用的遗传决定因素,在一个未损坏的小鼠皮肤感染模型中

Reid V Wilkening¹, Christophe Langouët-Astrié², Morgan M Severn³

¹Section of Pediatric Critical Care Medicine, Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA; Department of Microbiology and Immunology, University of Colorado School of Medicine, Aurora, CO 80045, USA.

|October 27, 2023

概括

斯氏菌 (Streptococcus pyogenes) 通过遗传适应感染小鼠皮肤,激活一个定数感应系统. 这个系统对于持续感染至关重要,增加疾病严重程度和炎症.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

杆菌 (Streptococcus pyogenes) 是一个重要的人类病原体,负责各种感染.
了解宿主-病原体相互作用对于开发有效治疗方法至关重要.

研究的目的:

开发一种小鼠模型来研究Streptococcus pyogenes上皮细胞感染.
研究S. pyogenes的遗传适应和感染期间宿主反应.
确定Rgg2/Rgg3定数感应系统在S. pyogenes皮肤感染中的作用.

主要方法:

开发了一种具有完整表皮质的小鼠模型,用于S. pyogenes感染.
在感染后5小时和24小时进行RNA测序,以分析宿主和细菌的基因表达.
实验性操纵Rgg2/Rgg3的质量检测系统.

主要成果:

细菌S. pyogenes经历了基因适应,包括代谢变化和Rgg2/Rgg3定数感应激活,以感染小鼠表皮.
完整的Rgg2/Rgg3定数感应级联对于建立稳定的表面皮肤感染至关重要.
控制人数感应系统的激活会增加小鼠的发病率,细菌负担,皮肤变化和炎症.

结论:

开发的小鼠模型有效地模拟了S. pyogenes的上皮相互作用.
在持续的S. pyogenes皮肤感染中,Rgg2/Rgg3定数感应途径至关重要.
准准决数感应系统可能是对抗S. pyogenes感染的治疗策略.

关键词:

CP: 微生物学微生物学在新西兰131 NZ131 在RNA-seqqq.在 Rgg2 / Rgg3 中.这是一种Streptococcus pyogenes.动物模型动物模型主机响应的主机响应.议员数量检测是指对议员数量进行检测.皮肤感染皮肤感染转录组 (transcriptome) 是一个转录组.

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