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Updated: Aug 11, 2026

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
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使用薄截面传输电子显微镜可视化病毒和人类血液单核细胞之间的甘氨酸介导结合的协议

Belinda L Spillings1, Kathryn Green2, Erica Lovas2

  • 1Institute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia.

STAR protocols
|October 27, 2023
PubMed
概括

这项研究详细介绍了一种传输电子显微镜 (TEM) 协议,以可视化病毒样颗粒 (VLPs) 和人类免疫细胞之间的甘氨酸介导结合. 这种方法有助于理解病毒附着和宿主细胞相互作用.

关键词:
细胞生物学 细胞生物学微生物学 微生物学显微镜的使用方法

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.