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Karolina Zygmunt¹, Agnieszka Otwinowska-Mindur², Katarzyna Piórkowska¹

¹Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.

Animals : an open access journal from MDPI

|October 27, 2023

概括

优化养殖肉类生产需要有效的牛卫星细胞 (BSC) 繁殖. 这项研究发现,牛血清和10 ng/mL的bFGF显著增强了BSC的增殖,而低葡萄糖支持关键的基因表达自我更新.

科学领域:

生物技术和食品科学生物技术和食品科学
细胞生物学和组织工程

背景情况:

预计到2040年,培养肉类生产将满足全球肉类需求的很大一部分.
成功的体外肌肉组织发育依赖于有效刺激卫星细胞的增殖,这是骨肌肉的前体.

研究的目的:

开发一种最佳的介质成分,以刺激牛类卫星细胞 (BSC) 的增殖.
研究葡萄糖度,血清类型和bFGF对BSCs的协同效应.

主要方法:

利用建模和优化技术来评估媒介组件效应.
测量了葡萄糖,血清 (牛与马) 和bFGF的协同作用和共同刺激作用.
在不同条件下使用qPCR分析基因表达 (Pax7,肌原性调节因子).

主要成果:

牛血清和10 ng/mL的bFGF显著增加了BSC增殖率.
根据血清类型或bFGF水平,没有观察到Pax7和MRF基因表达的显著差异.
低血糖介质导致Pax7和Myf5基因表达的统计显著增加 (p <0.05).

结论:

牛血清和10 ng/mL的bFGF是增强BSC增殖的关键因素.
介质中的低葡萄糖度对于维持参与卫星细胞自我更新的基因表达至关重要.

关键词:

牛的卫星细胞是牛的卫星细胞媒体构成媒体构成它们的扩散和扩散.

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