在酵母物种中通过Tra1控制醇反应的进化多样性
Gabriela Marsiglio Nunes Librais1, Yuwei Jiang1, Iqra Razzaq2
1Department of Anatomy and Cell Biology, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
G3 (Bethesda, Md.)
|October 27, 2023
概括
损害的Tra1蛋白功能通过调节抗性基因,增强了Candida albicans中的酸抗真菌药物耐受性,但降低了Saccharomyces cerevisiae中的耐受性,揭示了抗真菌反应的特定物种调节.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 遗传学 是一个遗传学.
背景情况:
- Tra1是酵母SAGA和NuA4复合物的关键协活性剂,通过基因素乙化调节基因表达.
- Tra1是一种PIKK家族的伪基因酶;突变 (tra1Q3) 损害了生长,增加了应激敏感性.
- 在Candida albicans中,tra1Q3降低了病原性,并增加了 echinocandin 抗真菌的敏感性.
研究的目的:
- 调查Tra1功能受损在酵母菌中抗真菌药物耐受性中的作用.
- 确定Tra1如何影响Candida albicans和Saccharomyces cerevisiae中对醇抗真菌剂的反应.
主要方法:
- 在Candida albicans和Saccharomyces cerevisiae中分析tra1Q3等位基因表型.
- 对抗醇基因的基因表达分析 (例如,ERG11,CDR1,PDR5).
- 测量亚醇抗真菌药物弗卢科纳的细胞内积累.
主要成果:
- 受损的Tra1功能 (tra1Q3) 增加了C. albicans中的醇抗真菌耐受性,提高了ERG11和CDR1表达的调节,并减少了可纳的积累.
- 相比之下,tra1Q3降低了S. cerevisiae中的醇耐受性,降低了PDR5的表达,增加了可纳的积累.
- 这些发现突出了Tra1在酵母菌种中对抗真菌药物反应的差异调节.
结论:
- Tra1在调节致病性和非致病性酵母的抗真菌药物耐药机制方面发挥了特定物种的作用.
- 了解Tra1的差异性功能可以为开发向抗真菌疗法提供信息.
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