相关实验视频

Updated: Jul 12, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

对Copan's ColibríTM的多中心评估,这是MALDI TOF MS目标应用程序的自动化仪器,用于细菌识别

My Lien Pham¹, Kenneth Van Horn¹, Ely Zarate²

¹Kaiser Permanente, Southern California Permanente Medical Group, Regional Reference Laboratories, Chino Hills, CA, USA.

Diagnostic microbiology and infectious disease

|October 27, 2023

概括

新的ColibríTM仪器自动选择细菌殖民地用于MALDI识别,显示99.54%与手工方法一致. 这种自动化提高了临床微生物学实验室的准确性和效率.

科学领域:

临床微生物学临床微生物学
在诊断的自动化.
质谱测量质量谱测量

背景情况:

手动选择殖民地用于MALDI识别是劳动密集型的.
准确和高效的细菌鉴定对于患者护理至关重要.
维特克® MS系统是基于MALDI的识别标准.

研究的目的:

为了比较自动化ColibríTM仪器的性能与使用VITEK® MS.进行细菌识别的手动检测.
评估ColibríTM系统的准确性,效率和可追溯性.

主要方法:

从各种临床样本 (尿液,伤口,呼吸道,血液) 中选择了殖民地.
科利布里TM自动取样,转移到MALDI目标幻灯片和矩阵叠加.
手动发现使用类似的殖民地进行了比较.
总共分析了444种细菌分离物.

主要成果:

在ColibríTM和手工方法之间存在很高的总体一致 (99.54%) (432/444个分离物被确定).
只有两个不一致的结果被观察到.
十二种有机体 (2.70%) 没有与ColibríTM识别.
科利布里TM在细菌识别方面表现出高准确度.

结论:

科利布里TM仪器为MALDI识别提供了准确和自动化的细菌殖民地选择解决方案.
使用ColibríTM的自动化可以通过重新分配技术人员时间来提高实验室效率.
该系统提供了从涂到识别的完整可追溯性,增强了实验室的工作流程.

关键词:

自动化自动化自动化马尔迪马尔迪马尔迪发现发现发现可追溯性可追溯性

更多相关视频

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Published on: May 28, 2014

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Published on: May 15, 2019

相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...