最近的发展是通过高性能液体色谱学来分离具有生物活性的性化合物的高通量分离
Chiara De Luca1, Simona Felletti2, Flavio Antonio Franchina1
1Department of Chemical, Pharrmaceutical and Agricultural Sciences, University of Ferrara, via L. Borsari 46, Ferrara 44121, Italy.
Journal of pharmaceutical and biomedical analysis
|October 27, 2023
概括
对生物活性化合物来说,评估体纯度至关重要. 嵌合体高性能液体染色学 (HPLC) 是关键的,近期在嵌合体静止相 (CSP) 中的进展使得高通量选的分离速度更快.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学是什么
- 制药科学 制药科学
背景情况:
- 生物活性化合物,包括活性药物成分 (API),通常是性的,其中立体异构体表现出明显的生物活性.
- 制造纯种分子化合物是具有挑战性的,通常会导致种族或的混合物.
- 对于监管合规和确保生物活性化合物的质量和安全而言,反体纯度评估至关重要.
研究的目的:
- 审查设计成功的高通量,快速的enantioseparations使用奇拉色谱的基本和实际方面.
- 讨论性静止阶段 (CSP) 的主要类别.
- 突出了近期在制药,法医,食品和电子领域的性分离的高通量应用.
主要方法:
- 状高性能液体染色学 (HPLC) 被提出为分离和净化反体的黄金标准.
- 讨论包括用于快速和超快速分离的性静止相 (CSP) 的开发.
- 考虑影响奇拉分离效率的实用和基本问题,包括粒子类型 (核心外与完全多孔).
主要成果:
- 奇拉尔HPLC提供了多功能性,由于广泛的商用CSP和操作模式.
- 最近的趋势集中在开发CSP,用于高吞吐量选的生物活性化合物.
- 在奇拉色谱中,核心外颗粒的动力优势并不总是保证的,需要仔细考虑.
结论:
- 成功的高通量反分离需要仔细考虑基本和实际的色谱特征.
- 该综述涵盖了关键的CSP类别和最近在不同领域的应用,这些领域需要奇拉分析.
- 在CSP技术的进步对于提高性分离的速度和效率至关重要.
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