基于板的LF-NMR/色度测量双模同质免疫传感器用于检测Vibrio parahaemolyticus
Dongyu Zhang1, Le Chen1, Han Lin1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
Food chemistry
|October 27, 2023
概括
一种新型的双模式免疫传感器使用低磁场核磁共振 (LF-NMR) 和色度检测检测出像Vibrio parahaemolyticus (VP) 这样的致病细菌. 这种方法为食品安全病原体检测提供了一个敏感和可靠的平台.
科学领域:
- 生物感知和诊断技术
- 纳米材料在分析化学中的应用
- 食品安全与微生物学
背景情况:
- 食品中的病原细菌对公众健康构成重大风险.
- 准确和快速的检测方法对于食品安全至关重要.
- 现有的检测技术可能缺乏灵敏度或需要复杂的程序.
研究的目的:
- 开发一种新的双模式免疫传感器,用于检测病原细菌.
- 为了提高灵敏度,利用低场核磁共振 (LF-NMR) 和色度测量.
- 建立一个可靠的平台来检测食物样本中的Vibrio parahaemolyticus (VP).
主要方法:
- 使用带有VP抗体和Fe3O4纳米粒子 (MNS@Ab2) 的石墨烯氧化物 (GO) 制造一个基于96孔板的免疫传感器.
- 在目标VP,捕获的抗体和信号单元之间形成免疫复合体.
- 酸溶解以创建同质系统,然后添加KMnO4用于LF-NMR和色度信号生成.
- 优化实验条件以最大限度地提高检测灵敏度和可靠性.
主要成果:
- 开发的免疫传感器显示,对VP的检测极限为60 CFU/mL.
- 双模式系统表现出良好的选择性,稳定性,精度,准确性和一致性.
- 使用LF-NMR和色度测量读数成功检测了致病细菌.
结论:
- 开发的LF-NMR/色度测量双模同质免疫传感器是用于检测致病细菌的简单可靠平台.
- 这种方法为加强食品安全监测提供了一个有希望的工具.
- 使用功能化纳米材料 (GO,Fe3O4) 显著提高了检测能力.
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