重建的卵巢在没有卵巢表面上皮质的情况下自组装
Enrique Sosa1, Sinthia Kabir Mumu1, Christian C Alvarado1
1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Center for Reproductive Science, Health and Education, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Stem cell reports
|October 27, 2023
概括
三维重建卵巢 (rOvary) 成功地用干细胞衍生的生细胞模型卵巢毛囊. 然而,这些模型缺乏像表面上皮质和theca这样的关键卵巢结构,限制了它们的生殖健康研究应用.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞研究 干细胞研究
- 3D 有机体模型
背景情况:
- 三维 (3D) 干细胞模型为推动妇女生殖健康研究提供了有前途的途径.
- 重建的卵巢 (rOvary) 是一个3D模型,它与多能干细胞衍生的生细胞自组,模仿卵巢功能.
- 这种模型支持毛囊内的功能性卵子细胞的分化.
研究的目的:
- 为了评估复制卵巢 (rOvary) 模型的细胞组成.
- 确定rOvary在模仿完整的卵巢结构方面的发展能力和局限性.
主要方法:
- 对rOvary模型细胞构成的分析.
- 在3D模型中评估毛囊发育和周围细胞相互作用.
主要成果:
- rOvary模型成功地产生了多个卵巢卵泡.
- 这些卵泡被NR2F2+流体细胞包围,表明了流体的发展.
- 关键的是,rOvary模型缺乏表面上皮质和类固醇的theca,这对成熟的卵巢功能至关重要.
结论:
- 重建的卵巢模型有效地回顾了从干细胞衍生组件中激活的卵泡的自我组装.
- 表面上皮质和theca的缺失意味着这些模型模仿了青春期前的卵巢,准备好,但尚未能够产生激素.
- 需要进一步精细化,以将这些缺失的细胞类型纳入更全面的生殖健康研究应用中.
相关概念视频
01:26Ovaries
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The ovaries are roughly the size of almonds and measure approximately 2 to 3 centimeters in length. These paired structures are situated within the pelvic region and are anchored by the mesovarium—a peritoneal extension that also connects them to the wider structure of the broad ligament. The support system extends to the suspensory ligament, housing blood and lymphatic vessels. In addition, the ovarian ligament tethers the ovaries to the uterus.
On the ovarian surface, a layer of...
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01:22Oogenesis
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Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
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01:27Ovarian Cycle
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The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
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01:20Folliculogenesis
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Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
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