培养,识别和检测肺非结核性菌根菌的抗菌素敏感性
Reeti Khare1, Barbara A Brown-Elliott2
1Mycobacteriology Laboratory, 1400 Jackson Street, National Jewish Health, Denver, CO 80238, USA.
Clinics in chest medicine
|October 27, 2023
概括
诊断非结核菌 (NTM) 肺部感染依赖于实验室检测. 显微镜具有较低的灵敏度,而培养方法提供识别,但具有较长的周转时间,需要更快的诊断方法.
科学领域:
- 微生物学 微生物学
- 临床诊断 临床诊断 临床诊断
- 传染性疾病 传染性疾病
背景情况:
- 非结核菌 (NTM) 是一种机会性病原体,会引起肺部感染.
- 准确的实验室诊断对于及时的疾病管理至关重要.
- 当前的诊断方法在速度和灵敏度方面存在挑战.
研究的目的:
- 审查目前用于NTM检测和识别的实验室方法.
- 讨论基于分子和蛋白质的测试的作用.
- 为了强调对NTM感染的敏感性测试的重要性.
主要方法:
- 显微镜用于快速酸性细菌的检测.
- 固体和液体培养用于NTM生长.
- 基于分子和蛋白质的测定用于NTM识别.
- 通过汁微稀释进行表型敏感性测试.
- 基因型敏感性方法,以获得更快的结果.
主要成果:
- 显微镜显示NTM检测的灵敏度较低.
- 培养方法是标准的,但耗时.
- 直接唾液测试提供更快的NTM识别.
- 标准化敏感性测试指南已经存在.
- 基因型方法可以加快抗菌素敏感性结果.
结论:
- 可靠的实验室结果对于诊断NTM肺部疾病至关重要.
- 更快的诊断方法,包括直接测试和基因型敏感性测试是有价值的.
- 建议遵守敏感性测试的既定指南.
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