帕尔瓦胺表达细胞和相邻的金字塔细胞之间的相互连接由聚类原素 γ 调节
Nanami Kawamura1, Tomoki Osuka1, Ryosuke Kaneko1
1KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
eNeuro
|October 27, 2023
概括
帕瓦胺阳性细胞中的聚类原cadherin-γ 调节了这些神经元如何与视觉皮层中的金字塔细胞连接. 这种基因删除会改变相互连接的平衡,影响神经电路功能.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 功能神经回路取决于刺激神经元和抑制神经元之间的精确连接.
- 特定神经元类型之间的突触合作伙伴识别的分子机制在很大程度上是未知的.
- 帕瓦胺阳性 (PV+) 细胞是皮层电路中的关键抑制性内内神经元.
研究的目的:
- 研究PV+细胞中聚类原cadherin-γ (cPcdhγ) 对于突触性伴侣识别的作用.
- 检查PV+细胞中cPcdhγ缺失对主要视觉皮层 (V1) 中的金字塔 (Pyr) 细胞的内层和跨层神经回路的影响 (V1).
- 阐明cPcdhγ如何影响PV+-Pyr微电路的互惠性.
主要方法:
- 全细胞记录和激光扫描光刺激与中的谷氨酸被用来绘制激发性输入的地图.
- 在对照和PV + 特定的cPcdhγ条件淘汰 (PV-cKO) 小鼠中进行了电生理学记录.
- 分析的重点是PV+和Pyr细胞之间的连接概率,互惠性和单元抑制后突触电流 (uIPSC) 幅度.
主要成果:
- 在PV+细胞中的cPcdhγ删除没有影响Pyr细胞的跨层激发性输入.
- 虽然总体PV+-Pyr连接概率没有变化,但PV-cKO小鼠的连接互惠率发生了显著的变化.
- 在PV-cKO小鼠中,具有较大的uIPSC振幅的PV+-Pyr对具有高度相互连接的比例减少,中等相互连接对的比例增加.
结论:
- 在PV+细胞中的聚类原cadherin-γ在调节皮质中的金字塔细胞与突触连接的互惠性方面发挥着至关重要的作用.
- cPcdhγ 影响PV+-Pyr细胞连接的特定模式,可能通过影响相互相互作用的强度或性质.
- 这些发现有助于理解在建立功能性皮质回路时突触伴侣选择的分子基础.
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