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使用光学光谱学快速量化生物固定

Haiyang Zhang^1,2, Jonathan M Plett², Karen L M Catunda²

¹College of Life Sciences, Hebei University, Baoding, China.

Journal of experimental botany

|October 27, 2023

概括

光学光谱学提供了一种快速,具有成本效益的方法来量化生物固定 (BNF). 该技术精确测量 (N) 和同位素,补充了BNF研究的传统方法.

科学领域:

农业科学农业科学
植物生理学植物生理学
生物地质化学生物地质化学

背景情况:

生物固定 (BNF) 是一个关键的全球输入,但其准确的量化是具有挑战性的.
传统的方法,如同位素比质谱法 (IRMS) 是耗时且昂贵的.
叶子反射光谱为评估植物状态提供了一个更快的替代方案.

研究的目的:

开发和验证一种使用光学光谱学量化BNF的新,快速和廉价的方法.
为了比较光学光谱学衍生的BNF测量与传统的IRMS量化.
评估该方法捕获BNF对环境变化的反应的能力.

主要方法:

使用IRMS,对来自受控和实地实验的植物组织进行了度 ([N]),同位素组成 (δ15N) 和来自大气固定 (Ndfa) 的N分析.
使用光学光谱学从相同的组织中预测相同的参数 ([N],δ15N,Ndfa).
对IRMS测量进行了验证,对光谱预测进行了验证.

主要成果:

与IRMS相比,光学光谱学准确地预测了Ndfa,具有高的R2值 (温室中为0.90,现场为0.94).
该方法与传统量化方法有很强的一致性,斜率接近1.
预测豆类 δ15N 的根平均平方误差很低 (0.38 和 0.43).

结论:

光学光谱学提供了一个快速,可扩展和具有成本效益的工具来量化BNF.
这种技术补充了现有的方法,并促进了更广泛的BNF研究.
该方法有效地捕捉了气候变化场景 (如变暖和干旱) 下的BNF动态.

关键词:

的同位素组成构成.气循环中的气循环.空间变化的空间变化.共生固定是一种共生固定.时间变化的时间变化.δ15NN 15NN 的时间.

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