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研究微RNA作为意识障碍中的生物标志物:一个纵向的多中心研究

Nicolò Musso¹, Dalida Bivona¹, Carmelo Bonomo¹

¹Department of Biomedical and Biotechnological Sciences, University of Catania, 95123, Catania, Italy.

Scientific reports

|October 27, 2023

概括

特定的microRNAs (miRNAs) 在长期意识障碍 (DoC) 中显示变化的水平. 在创伤性脑损伤患者中,miRNAs 132-3p和23b-3p可以预测更好的结果.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

微RNAs (miRNAs) 调节基因表达,并影响患有意识障碍 (DoC) 的患者的二次脑损伤和恢复.
了解miRNA的作用对于改善DoC患者的预后和治疗策略至关重要.

研究的目的:

调查五种特定的miRNAs (150-5p,132-3p,23b-3p,451a和16-5p) 在长时间的DoC中的作用.
探索miRNA水平与病因因素 (创伤性脑损伤 (TBI) 与缺氧缺血性脑损伤 (HIBI)) 和患者结果之间的关联.

主要方法:

来自30名DoC患者的血清样本 (不响应的清醒综合征或最小意识状态) 在基线 (1-3个月) 和受伤后6个月的miRNA水平上进行了分析.
患者的诊断得到了证实,使用昏迷恢复量表-修订.
在6个月后,使用格拉斯哥结果扩展量表 (GOSE) 和功能独立度量 (FIM) 评估了功能结果.

主要成果:

与对照组相比,TBI患者的miRNAs150-5p,132-3p和23b-3p的基线水平较低.
患有HIBI的患者在基线时表现出较低的miRNA 150-5p和减少的miRNA 451a水平,在6个月后持续低的miRNA 150-5p.
在TBI患者中,miRNAs 132-3p和23b-3p的升高水平与更好的GOSE和FIM得分相关.

结论:

在长期的DoC中观察到明显的miRNA失调模式,因病因和受伤后的时间而异.
MiRNAs 132-3p和23b-3p显示出作为TBI相关的DoC中恢复的预后生物标志物的潜力.
需要进一步的研究来阐明这些miRNAs在脑损伤和恢复中的精确机制.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...