慢性骨髓炎中致病性微生物的组成基于元基因组测序及其在病因学诊断中的应用价值
Kang Zhang1, Yu-Zhe Bai2, Chang Liu3
1Laboratory Medicine of Beijing Tongren Hospital affiliated to Capital Medical University, Beijing, China.
BMC microbiology
|October 27, 2023
概括
与传统培养方法相比,甲基因组下一代测序 (mNGS) 在慢性骨髓炎中提供了无氧细菌和Mycobacterium结核病的优越检测. 这种先进的技术增强了病因诊断,并指导复杂的骨感染的治疗策略.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 慢性骨髓炎的诊断传统上依赖于低检测率的培养方法.
- 这种限制使得有效的感染治疗指南复杂化.
- 下一代元基因组测序 (mNGS) 为微生物检测带来了潜在的进步.
研究的目的:
- 为了阐明慢性骨髓炎的致病性微生物组成.
- 将mNGS的检测率与标准培养方法进行比较.
- 探索mNGS在慢性骨髓炎病因诊断中的实用性.
主要方法:
- 分析了来自慢性骨髓炎患者的50个手术内骨组织样本.
- 样品进行了基因组下一代测序 (mNGS) 和传统微生物培养.
- 通过将临床数据与检查结果相结合来确定致病原体.
主要成果:
- mNGS检测出比培养更多的无氧细菌和Mycobacterium结核病 (MTB) 菌株.
- 在mNGS和培养之间,总体阳性检测率没有显著差异.
- mNGS显示无氧细菌和MTB的检测率显著更高 (P<0.05).
结论:
- mNGS增强了对慢性骨髓炎中微生物分布的理解.
- 培养方法对于有氧细菌,真菌和药物敏感性测试仍然很有价值.
- mNGS在厌氧细菌,MTB和混合感染方面表现出卓越的性能,指导了改进的实验室诊断.
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