转录学与代谢学相结合,揭示了寒冷应激对大米微胞子的影响
Yingbo Li1,2, Yingjie Zong1,2, Wenrui Li1,2
1Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
BMC plant biology
|October 27, 2023
概括
冷处理通过重编程代谢途径来增强大米微粒的全能性. 关键的分子事件,包括氨基酸和碳水化合物新陈代谢的转变和酸的参与,对于微胞胚胎发生的启动至关重要.
科学领域:
- 植物生物技术 植物生物技术
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 微胞培养对于植物育种至关重要,但微胞胚胎生成诱导效率各不相同.
- 寒冷处理可以重编程细胞细胞形成途径,但其在微胞胚胎生成中的精确分子机制尚不清楚.
研究的目的:
- 为了研究寒冷压力对大米微粒的强度的影响.
- 阐明了冷诱导微胚胎生成背后的分子机制.
主要方法:
- 米品种中华十一号经过冷处理.
- 用RNA测序 (RNA-seq) 来进行差异基因表达 (DEG) 分析.
- 气色谱-质谱 (GC-MS) 和液色谱-质谱 (LC-MS) 用于差异代谢物表达 (DEM) 分析.
- 综合转录学和代谢学分析.
主要成果:
- 10天的冷治疗对于微粒子胚胎发生的启动至关重要,在治疗后7天的微粒子存活率达到峰值.
- 寒冷压力诱导了基因表达的显著变化,基因本体学 (GO) 术语从"对非生物刺激的反应"转变为"代谢过程",特别是涉及"细胞壁".
- 代谢分析揭示了更多的下调代谢物,主要是"脂质和类似脂质的分子",KEGG途径分析表明氨基酸,核酸和碳水化合物代谢发生了改变. 综合分析强调"氨基酸代谢"和"碳水化合物代谢"是关键途径,而酸被确定为潜在的关键代谢物.
结论:
- 寒冷压力通过分子重编程显著影响大米微粒的全能性.
- "氨基酸代谢"和"碳水化合物代谢"的途径对于微胞胚胎生成至关重要.
- 利酸可能在冷应力下启动微胞胚胎生成中发挥关键作用.
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