影响葡萄藤细胞培养在诱导下对白醇生物生产的因素
Juan Carlos Vera-Urbina1, Susana Sellés-Marchart1, Ascensión Martínez-Márquez1
1Departamento Bioquímica y Biología Molecular y Edafología y Química Agrícola, Facultad de Ciencias, Universidad de Alicante, 03690 Alicante, Spain.
Biomolecules
|October 28, 2023
概括
这项研究优化了葡萄细胞培养中使用甲基斯酸盐 (MeJA) 和二甲基-β-环德 (DIMEB) 的转白 (t-R) 生产. 确定了在生物反应器中增强t-R产量的最佳条件.
科学领域:
- 植物生物技术 植物生物技术
- 自然产品的合成自然产品的合成
- 生物工艺工程 生物工艺工程
背景情况:
- 葡萄细胞培养为转白 (t-R) 生产提供了一个可持续的来源.
- 优化诱导策略对于最大限度地提高植物细胞生物生产系统中的t-R产量至关重要.
研究的目的:
- 描述和优化Vitis vinifera细胞培养中的转白醇 (t-R) 的产生.
- 确定影响t-R生产的关键因素,以成功升级到生物反应器.
主要方法:
- 研究了生物质密度,诱导剂度 (MeJA,DIMEB) 和添加顺序的影响.
- 评估了照明模式,细胞衰老和生物反应器条件 (空气,温度,糖糖).
主要成果:
- 在19%的生物质密度和0.5g DIMEB/g生物质比率下实现最佳的t-R生产.
- 使用50毫米DIMEB和100微米MeJA获得4.18毫克/毫升t-R和16.3毫克/克生物质的峰值度.
- 在DIMEB前48小时添加MeJA降低了t-R收益率;在DIMEB后48小时添加MeJA时没有观察到显著差异.
结论:
- 在葡萄藤细胞培养中建立了t-R生产的最佳参数.
- 证明了将t-R生物生产扩展到混合生物反应器的可行性,并保持了效率.
关键词:
葡萄酒 (Vitis vinifera) 是一种葡萄酒.生物生产生物制造生物反应器是一个生物反应器.甲基斯蒙酸盐是什么甲基-β-循环德克斯特林.植物细胞培养植物细胞培养.复星醇是一种复星醇.尽管如此,Stilbene仍然存在.更多相关视频
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