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在纳米结构的等离子表面上对DNA杂交的研究,用于识别鼻病毒

Shao-Sian Li¹, Yi-Jung Lu², Ray Chang³

¹Department of Materials and Mineral Resources, National Taipei University of Technology, Taipei 10608, Taiwan.

Bioengineering (Basel, Switzerland)

|October 28, 2023

概括

一个新的设备可以同时检测SARS-CoV-2和爱斯坦-巴尔病毒 (HHV-4) DNA. 这种快速,无标签的检测方法有助于识别共感染,并评估重症COVID-19病例的治疗方法.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
传染病研究传染病研究

背景情况:

人类疹病毒4 (HHV-4),或爱斯坦-巴尔病毒,与SARS-CoV-2感染的严重程度增加有关.
与HHV-4和SARS-CoV-2的同时感染与发烧,炎症和死亡率的风险更高相关.
现有的SARS-CoV-2诊断工具通常依赖于基于标签的检测,限制直接产品测量.

研究的目的:

开发一种用于同时复制和检测SARS-CoV-2和HHV-4DNA的新型装置.
通过与微流体PCR集成的基于等离子体的传感器实现病毒DNA的无标签检测.
建立一种更快,更灵敏的病毒共感染诊断方法.

主要方法:

开发用于双重聚合酶链反应 (PCR) 的微流体装置.
基于等离子体的传感器的实施,用于检测PCR amplicons的非标记检测.
使用人工感染HHV-4/SARS-CoV-2的细胞测试设备的性能.

主要成果:

与传统仪器相比,该设备实现了55%的PCR时间缩短,具有可比的安普利康产量.
显示了100 fg/mL的检测极限 (LOD),明显低于以前的非标记传感器 (ng/mL到pg/mL).
在人工共感染的细胞中成功检测出目标基因.

结论:

开发的设备提供了一种快速,敏感和无标签的方法来检测SARS-CoV-2和HHV-4共感染.
这项技术可以帮助临床识别HHV-4/SARS-CoV-2共感染患者.
该设备具有评估共感染个体的实际治疗策略的潜力.

关键词:

这就是SARS-CoV-2病毒.双重PCR是一种双重PCR.人类疹病毒4 没有标签的无标签.基于纳米切割的塑材料聚合酶连锁反应的发生.

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