新型基因工程益生菌用于在肠道殖民中向消除Pseudomonas aeruginosa

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Hyun Kim¹, Ju Hye Jang¹, In Young Jung²

¹Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea.

|October 28, 2023

概括

工程益生菌通过释放特定的抗微生物来检测和消除Pseudomonas aeruginosa. 这种向治疗可以减少肠道中的P. aeruginosa,为易受伤害的患者提供了一种新方法.

科学领域:

微生物学微生物学
合成生物学合成生物学
生物技术是生物技术.

背景情况:

Pseudomonas aeruginosa 的肠道携带在免疫受损和住院患者中很高,增加了感染风险.
与抗生素相关的腹在这些患者群体中是一个重大问题.
自主抗菌疗法为向消除病原体提供了一个有希望的解决方案.

研究的目的:

开发用于检测和根除Pseudomonas aeruginosa的基因工程益生菌.
创建一个系统,其中益生菌生产和分泌Pseudomonas aeruginosa选择性抗微生物 (AMP) 的需求.

主要方法:

构建基于等离子体的系统,用于AMP的产生和分泌,以应对P. aeruginosa的定数感应分子 (3OC12HSL).
利用微信V分泌系统或YebF作为AMP分泌的载体蛋白.
转移到Escherichia coli Nissle 1917 (EcN) 的系统,并在试验室和小鼠模型中进行了测试.

主要成果:

工程 EcN 成功表达和分泌PA2-GNU7,在体外抑制P. aeruginosa的生长.
施用工程 EcN 降低了小鼠便和结肠中的 P. aeruginosa 水平.
证明了工程 EcN 在打击肠道 P. aeruginosa 殖民化的有效性.

结论:

经过基因工程改造的EcN证明了针对P. aeruginosa的自主抗菌疗法的潜力.
这种方法可以减少肠道P. aeruginosa的殖民化,减轻易受感染患者的感染风险.
工程益生菌提供了一个有针对性和按需的治疗策略.

关键词:

大肠杆菌 Escherichia coli 尼斯尔 1917 年伪omonas aeruginosa 这种类型的病毒.抗微生物是一种抗微生物.抗微生物耐药性抗微生物耐药性工程制造的益生菌是一种生物.

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