撤回:梅拉在良性乳腺细胞中的潜在保护作用,这些细胞被恶性细胞的细胞外囊重新编程
Caroline Procópio de Oliveira1,2, Barbara Maria Frigieri1,3, Heidge Fukumasu4
1Cancer Molecular Research Laboratory (LIMC), Faculdade de Medicina de São José do Rio Preto-FAMERP, Av. Brigadeiro Faria Lima, São José do Rio Preto 15090-000, SP, Brazil.
Biomedicines
|October 28, 2023
概括
狗乳腺瘤外体促进癌症生长,但黑激素可以抵消这些影响. 这项研究强调了黑激素.
科学领域:
- 兽医瘤学 兽医瘤学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 狗乳腺瘤作为人类乳腺癌的宝贵临床前模型.
- 细胞外囊泡 (EVs),特别是外体,通过调解细胞间通信,在乳腺癌的进展中发挥着至关重要的作用.
- 黑激素是一种具有已知的抗瘤特性的激素,正在研究其在癌症治疗中的潜力.
研究的目的:
- 研究来自犬乳腺瘤的外体对良性上皮细胞的影响.
- 评估黑激素在调节外体诱导瘤进展中的治疗潜力.
- 探索对mTOR信号通路的影响.
主要方法:
- 从乳腺瘤的狗的血中分离出了EVs.
- 良性犬类上皮细胞 (E-20) 与EVs和/或黑激素共同培养.
- 测试包括mTOR和AKT蛋白质的殖民地形成,增殖,迁移和免疫组织化学.
主要成果:
- 狗乳腺瘤衍生的外体显著增强细胞增殖,迁移和殖民地形成.
- 外基因组上调调节了mTOR和AKT蛋白的表达.
- 黑色素治疗显示出一种保护作用,抵消了外体细胞的前瘤性作用.
结论:
- 狗乳腺瘤的外体通过mTOR途径促进瘤的进展.
- 黑素可以调节EV介导的信号传输,在兽医瘤学中提供潜在的治疗策略.
- 这项研究支持使用黑激素来治疗狗乳腺瘤.
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