优化微波处理和酶提取的整合,以生产丰富的多醇提取物从橄树
Gabriela A Macedo1,2, Paula de P M Barbosa1, Fernanda F G Dias2
1Bioprocesses Laboratory, DEPAN/FEA (School of Food Engineering), Unicamp (University of Campinas), R. Monteiro Lobato, 80, Campinas 13083970, Brazil.
Foods (Basel, Switzerland)
|October 28, 2023
概括
像微波和酶辅助提取 (MEAE) 等绿色技术增强了从橄果汁中提取生物活性化合物的能力. 使用酶或联合酶优化MEAE显著提高了和抗氧化剂的产量,提供了一种具有成本效益和环保的方法.
科学领域:
- 农业和食品化学 农业和食品化学
- 绿色化学 绿色化学
- 生物技术是生物技术.
背景情况:
- 橄油是富含生物活性化合物的丰富来源.
- 传统的提取方法往往是低效的,对环境造成负担.
- 绿色技术为生物活性化合物回收提供了可持续的替代方案.
研究的目的:
- 优化微波和酶辅助提取 (MEAE) 来从橄果汁中提取和抗氧化剂化合物.
- 为了评估不同酶组合 (单独的tannase与tannase,cellulase和pectinase) 对提取效率的影响.
- 确定最大限度地提高生物活性化合物产量和抗氧化活性的最佳条件.
主要方法:
- 使用微波和酶辅助提取 (MEAE).
- 使用中央复合可旋转设计来优化提取参数 (酶度,温度,果/水比).
- 使用二极管阵列检测 (HPLC-DAD) 的高性能液体染色学用于化合物分析.
主要成果:
- 使用酶优化的MEAE产生了7110.6毫克GAE/kg,而使用联合酶的MEAE产生了2938.25毫克GAE/kg.
- 提取效率取决于酶,化酶有利于酸和联合酶产生氧醇,酸和酸.
- 抗氧化活性与释放的酸相关.
结论:
- 综合MEAE是一种有效和高效的方法,用于从橄果汁中提取生物活性.
- 这种绿色技术方法减少了加工时间和成本,为农业工业副产品的价值化提供了一个可持续的替代方案.
- 酶选择显著影响了提取物的形状和抗氧化能力.
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