用气色谱-质谱法 (GC-MS/MS) 确定农产品中甲分析的修改QuEChERS方法
Da-Young Yun1, Ji-Yeon Bae1, Chan-Woong Park2
1Food Safety Evaluation Department, Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju 28159, Republic of Korea.
Foods (Basel, Switzerland)
|October 28, 2023
概括
使用修改后的QuEChERS和GC-MS/MS的新方法有效地检测到农作物中的甲醇杀菌剂残留物. 这种分析通过监测农产品中持久性有机杀菌剂来确保食品安全.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 甲尼尔是一种有机类杀菌剂,在农业中广泛用于预防真菌病.
- 它在农产品中的持久性需要可靠的分析方法来监测残留物.
- 现有的方法可能不够灵敏或高效,无法对甲进行常规分析.
研究的目的:
- 开发和验证一种可靠的分析方法,用于确定农产品中的甲基残留物.
- 建立高效的提取和净化程序,以准确量化.
- 评估该方法是否适用于韩国共和国的例行监测和监管合规性.
主要方法:
- 经过修改的QuEChERS (快速,轻松,廉价,有效,坚固,安全) 提取方法与气相色谱法-质谱法/质谱法 (GC-MS/MS) 相结合.
- 优化提取溶剂 (乙二与/酸) 和分散固相提取 (d-SPE) 清洁 (PSA,C18).
- 使用五种农产品 (棕,,大豆,胡,马) 的方法验证,包括线性,LOD,LOQ,回收和精度研究.
主要成果:
- 优化的方法显示出极好的线性 (R2 = 0.9939-0.997) 和低检测极限 (LOD = 0.003 mg/kg) 和量化极限 (LOQ = 0.01 mg/kg).
- 平均恢复率从79.3%到104.1%不等,变化系数<17.9%,表明高准确度和精度.
- 在十种农产品中检测到甲的残留物,包括果,西瓜和黄瓜,证实了该方法的适用性.
结论:
- 开发的QuEChERS-GC-MS/MS方法非常有效,适合在各种农产品中常规检测甲残留物.
- 该方法的LOQ符合积极清单系统 (PLS) 的要求,支持监管食品安全标准.
- 这些发现提供了关键数据,以告知和改进当前关于杀真菌剂残留物管理的食品政策.
相关概念视频
01:14Gas Chromatography–Mass Spectrometry (GC–MS)
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01:21Mass Spectrometry: Complex Analysis
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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