精液分娩期间的温度升高通过促进细胞亡来降低野猪精子质量
Junwei Li1,2, Wenming Zhao1,2, Jiaqiao Zhu1,2
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Animals : an open access journal from MDPI
|October 28, 2023
概括
在野猪精液输送过程中温度升高会损害精子质量和运动性,即使在冷却后也是如此. 热冲击蛋白HSP70和HSP90可以通过激活AMPK和减少亡来帮助精子存活.
科学领域:
- 动物繁殖 动物繁殖
- 精子生物学 精子生物学
- 分子生物学分子生物学
背景情况:
- 猪人工授精依赖于高质量的野猪精子输送.
- 在温暖的条件下进行精子输送可能会损害精子的生存能力和生育能力.
研究的目的:
- 评估精液分娩期间高温对野猪精子质量和功能的影响.
- 调查分子机制,包括亡和蛋白质表达,参与精子对热应激的反应.
主要方法:
- 猪精液经过模拟的分娩条件 (37°C24小时在聚烯盒中),然后冷藏 (17°C).
- 精子质量 (流动性),细胞灭绝标记物 (Bax,Caspase 3),化AMPK和热冲击蛋白 (HSP70,HSP90) 通过CASA,流细胞计和西式斑点分析.
主要成果:
- 升高的温度在14小时后显著降低了野猪精子的质量和运动性,冷却后没有恢复.
- 热应激增加了精子亡 (Bax,Caspase 3) 并改变了AMPK酸化.
- 随着温度的上升,HSP70和HSP90的表达显著增加.
结论:
- 在野猪精液分娩期间的温度升高严重损害了精子质量和功能,通过诱导亡.
- HSP70和HSP90可能在猪精子耐热性中发挥作用,可能是通过AMPK激活和抗亡途径.
相关概念视频
Spermatogenesis
102.6K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.6K
Factors Affecting Body Temperature
4.3K
As a nurse, it is vital to understand the factors affecting body temperature to monitor variations and effectively evaluate deviations from regular.
Factors may include:
Factors may include:
4.3K
Sperm Structure and Semen Composition
2.4K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
2.4K
Sperm Transport
866
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
866
Testes: Histology
962
A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
The spermatogenic cells, responsible for producing sperm, are...
962
Increased Body Temperature
685
A body temperature above 38°C (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
685


