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黑色素通过加强它们的抗氧化防御来保护牛精子

Sophia N Lavrentiadou¹, Vasiliki Sapanidou¹, Elena E Tzekaki²

¹Laboratory of Physiology, Department of Animal Structure and Function, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Animals : an open access journal from MDPI

|October 28, 2023

概括

补充黑素可以提高冷保存的牛精子的质量. 这种抗氧化激素在解后增强了精子的运动性,活力和抗氧化能力,为辅助生殖技术提供了好处.

科学领域:

生殖生物学生殖生物学
生物化学生物化学
动物科学动物科学

背景情况:

精液的冷保存对于辅助生殖技术至关重要.
脱后的精子因活性氧物种和抗氧化剂枯竭而面临氧化应激.
在精子制备介质中补充抗氧化剂是减轻损伤的常见策略.

研究的目的:

为了研究黑激素在处理过程中对冷保存的牛精子的保护作用.
评估黑激素在正常和氧化应激条件下对精子质量的影响.

主要方法:

被冷保存的牛精子被解并与100μM的黑素或没有化.
精子被挑战用100微米的过氧化来模拟氧化应激.
用计算机辅助精子分析 (CASA) 评估精子质量,用于动力学,试蓝色染色用于生命力,以及谷氨酸/NBT测定和抗氧化状态 (谷氨酸和iNOS水平) 的西部斑.

主要成果:

黑色素显著改善了精子运动参数,增加了运动和快速精子的百分比.
在有黑激素的情况下,精子活力更高.
通过增加细胞中的谷氨水平和降低可诱导的氧化合成酶 (iNOS) 蛋白质水平,黑素增强了抗氧化剂状态.

结论:

黑色素作为一种强有力的抗氧化剂,保护牛精子细胞免受冷保存引起的损伤.
补充黑素可以提高解精子的质量和活力,使其成为辅助生殖中精子制备介质的有希望的添加剂.

关键词:

抗氧化能力的抗氧化能力.葡萄糖氨酸氨酸是什么?过氧化是一种过氧化.氧化合成酶是一种氧化合成酶.氧化应激是一种氧化应激.有活性氧物种的反应性氧物种.精子生理学精子生理学超氧化物离子超氧化物离子

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