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在肉类中识别影响脂肪相关肉类特征的功能基因,使用双消化限制相关的DNA测序和分子对接分析

Siyu Yuan¹, Shaoqi Tian¹, Chuang Meng^1,2

¹College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.

Animals : an open access journal from MDPI

|October 28, 2023

概括

研究人员确定了19个候选基因,影响子肉中的脂肪沉积,特别关注ACAA1,ACAA2和ACACB基因. 这些基因对脂肪酸代谢至关重要,并可能解释子品种之间的肉质差异.

科学领域:

动物遗传学动物遗传学
营养基因组学营养基因组学
肉类科学肉科学

背景情况:

石 (SQ) 和白王 (WK) 是中国经济上重要的肉品种.
在SQ和WK子之间,肌肉内脂肪 (IMF) 含量和乳房肉的脂肪酸 (FA) 概况存在显著差异.

研究的目的:

在肉类子中确定负责脂肪相关肉质特征的候选基因.
调查与IMF和FA组成的差异相关的基因组变异.

主要方法:

双消化限制关联DNA (ddRAD) 测序在12只 (SQ和WK) 上进行.
用基因本体学和通路分析来选候选基因.
用分子对接来评估特定遗传变异对酶基质相互作用的影响.

主要成果:

包括ACAA1,ACAA2和ACACB在内的19个候选基因被确定为可能影响与脂肪相关的特征.
这些基因在脂肪酸代谢,降解和生物合成途径中得到显著丰富.
发现ACAA1,ACAA2和ACACB中的特定非同义变化可能会影响酶基质结合和催化效率.

结论:

ACAA1,ACAA2和ACACB中的SNP需要进一步研究它们在子脂肪相关特征变异中的作用.
了解这些遗传因素可以帮助改善商业子品种的肉质特征.

关键词:

候选基因是指候选基因的基因.在 ddRAD 测序中使用 ddRAD 测序.脂肪酸脂肪酸是一种脂肪酸.肌肉内脂肪肌肉内脂肪分子对接分析分子对接分析牛是什么意思牛是什么意思

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