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绵羊的适应反应:不同皮质醇集群中的母羊揭示了唾液miRNAs表达的变化

Isabella Manenti¹, Irene Viola¹, Ugo Ala¹

¹Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.

Animals : an open access journal from MDPI

|October 28, 2023

概括

唾液微RNAs (miRNAs) 在母羊中显示出不同的表达模式,反映了压力反应. 这些生物标志物可以帮助识别具有不同应激适应特征的绵羊,以改善农场动物福利.

科学领域:

动物科学动物科学
分子生物学分子生物学
兽医医学兽医医学兽医医学

背景情况:

农场程序激活下丘脑-垂体-上腺轴,通过生理变化影响动物福利.
微RNAs (miRNAs) 对于平衡至关重要,并且正在成为动物中与压力有关的疾病的生物标志物.
唾液中循环的miRNA提供了一种非侵入性的方法来评估动物的应激反应.

研究的目的:

在羊唾液中表达的特定微RNA (miRNA) 的识别和特征.
调查唾液miRNA表达模式与皮质醇指示的压力水平之间的关系.
探索唾液miRNAs作为生物标志物的潜力,以评估农场绵羊的应激适应.

主要方法:

在20只母羊中对唾液皮质醇趋势和水平进行集群分析.
唾液样本采集时间与常见的农场实践 (引入公羊).
16个与压力相关的miRNA的选择和放大,重点是miR-16b,miR-21,miR-24,miR-26a,miR-27a,miR-99a和miR-223.

主要成果:

根据皮质醇概况,确定了两种不同的雌羊群.
在皮质醇集群之间观察到miR-16b和miR-21表达的显著差异 (p <0.05).
趋势分析显示了miRNA表达的聚类 (p = 0.017),表明miR-16b和miR-21在综合应激反应中的作用.

结论:

这项研究是第一个确定绵羊唾液中的特定miRNA及其与基于皮质醇的压力集群相关的差异表达的研究.
唾液miR-16b和miR-21显示出作为绵羊综合应激反应的生物标志物的潜力.
评估唾液miRNA可以帮助选择具有不同应激反应概况的母羊,从而有助于改善农场管理和动物福利.

关键词:

动物福利动物福利在循环中的miRNA.这是唾液,唾液.羊群羊群羊群的羊群是什么意思压力反应应对压力的反应.

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