不同封装技术在模拟胃条件下不同菌体的活力和稳定性的有效性
Sicelo B Dlamini1, Adriano M Gigante2, Steven P T Hooton3
1School of Agricultural Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga, Nelspruit 1200, South Africa.
Microorganisms
|October 28, 2023
概括
保护沙门氏菌菌体免受苛刻的胃肠道的影响,是有效的牲畜治疗的关键. 含有碳酸 (CaCO3) 的酸-卡拉基南微囊在保护这些病毒方面表现有前途.
科学领域:
- 微生物学 微生物学
- 兽医科学 兽医科学 兽医科学
- 生物技术是生物技术.
背景情况:
- 沙门氏菌感染给人类和牲畜带来了重大的公共卫生和经济挑战.
- 沙门氏菌的抗微生物耐药性正在增加,这是由于密集农业广泛使用抗生素造成的.
- 菌体为减少家畜沙门氏菌的抗生素提供了一个有希望的替代品.
研究的目的:
- 评估阿尔金酸盐 - 卡拉基南微囊在保护沙门氏菌菌体免受模拟胃肠道疾病的有效性.
- 评估碳酸 (CaCO3) 含量对菌体活力和释放的影响.
- 确定封装细菌菌体在单胃动物中具有针对性肠道输送的潜力.
主要方法:
- 开发含有或不含CaCO3的酸盐-氨酸微囊,以封装五种不同的沙门氏菌菌体.
- 封装和未封装的菌体在不同时间内暴露于模拟的胃条件 (低pH,酶) 中.
- 在模拟消化后使用斑块试验 (PFU/mL) 评估菌体活力.
- 在中性pH值下 (模拟肠道状况) 从微囊释放菌体的评估.
主要成果:
- 在pH值<3.0的10分钟后,未受保护的菌体没有恢复.
- 酸-氨酸封装在pH 2-2.5下改善了菌体的存活时间,持续了10分钟.
- 加入1%的CaCO3显著增强了菌体的保护,在pH 2下在1小时后<0.5 log10 PFU/mL损失.
- 从微囊中观察到,当pH值转移到7.5时,可以有效地释放菌体.
结论:
- 酸 - 卡拉基南微囊为沙门氏菌菌提供了必要的保护,防止严重的胃肠道疾病.
- 添加CaCO3进一步优化了模拟消化环境中的菌体活力和保护.
- 这种封装策略代表了一种可行的方法,用于在牲畜中向肠道输送沙门氏菌菌体,潜在地打击抗菌素耐药性.
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