在长期样本保存后,对本地和侵入性淡水双动物的肠道微生物群落进行表征
Stephanie N Vaughn1, Carla L Atkinson2, Paul D Johnson3
1Department of Biology, University of Mississippi, University, MS 38677, USA.
Microorganisms
|October 28, 2023
概括
淡水的乙醇保存是研究它们的肠道微生物组的有效方法. 博物馆藏品可以用来跟踪肠细菌随时间变化的变化.
科学领域:
- 生态生态学 生态生态学
- 微生物学 微生物学
- 保护科学 保护科学
背景情况:
- 淡水是重要的环境健康指标,但由于息地丧失,气候变化和污染,它们的数量正在下降.
- 贝类生物多样性下降对淡水生态系统构成风险,突出强调需要了解它们的肠道微生物组,以保护它们.
- 博物馆藏品为历史生态数据提供了潜在的资源,但保存的标本对于微生物组分析的适用性尚不清楚.
研究的目的:
- 为了确定以乙醇保存的淡水标本是否为历史生态研究提供了代表性肠道微生物群数据.
- 通过使用侵袭性鱼*Corbicula fluminea*进行双微生物组分析,验证乙醇作为一种保存方法.
- 评估9种本地鱼物种在乙醇中保存2-9年的肠道微生物组.
主要方法:
- 使用*Corbicula fluminea*进行了一项保存实验,以测试乙醇对微生物群保存的有效性.
- 在乙醇中储存2至9年的9种本土贝类的肠道微生物组使用测序进行了分析.
- 在新鲜和保存样本之间比较了细菌组合和测序深度.
主要成果:
- 乙醇保存有效地保持了双肠样本的测序深度和细菌丰富度.
- 这项研究提供了对*C. fluminea*和9种本土菌种的肠道微生物的见解.
- 一个核心细菌属,Romboutsia,被确定为可能在淡水双鱼物种中很常见.
结论:
- 乙醇是保存淡水标本的合适方法,用于肠道微生物组研究.
- 博物馆收藏的以乙醇保存的贝可以用来研究它们的肠道微生物组的长期变化.
- 识别像Romboutsia这样的核心细菌属有助于了解的健康和生态作用.
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