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Updated: Jul 12, 2025

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欧洲废水监测非脊髓灰质炎肠道病毒及其他疾病

Laura Bubba¹, Kimberley S M Benschop², Soile Blomqvist³

¹European Non-Polio Enterovirus Network (E.N.P.E.N.), 1207 Geneva, Switzerland.

|October 28, 2023

概括

废水监测 (WWS),最初是针对脊髓灰质炎病毒,现在监测非脊髓灰质炎肠道病毒 (NPEV) 和其他病原体. 这种灵活,基于人口的工具使用不断发展的方法,包括下一代测序,以可靠地检测疫情爆发.

科学领域:

环境微生物学环境微生物学
公共卫生病毒学.
分子流行病学分子流行病学

背景情况:

废水监测 (WWS) 起源于20世纪60年代,用于检测脊髓灰质炎病毒.
非脊髓灰质炎肠道病毒 (NPEV) 越来越多地被认为是儿童发病的原因.
在将WWS应用于新病原体时,方法的适用性至关重要.

研究的目的:

讨论WWS对非脊髓灰质炎肠道病毒 (NPEV) 的历史,方法和应用.
探索WWS从基于培养的到基于分子和测序的检测的演变.
考虑未来在WWS中实施下一代测序 (NGS).

主要方法:

由欧洲非脊髓灰质炎病毒网络 (ENPEN) 组织的专家网络研讨会.
在污水样本中检测脊髓灰质炎病毒的模拟实验的讨论.
对基于培养,基于分子和基于序列的病原体检测试验的审查.

主要成果:

模拟实验表明400毫升的污水样本足以检测小儿麻症病毒,分泌率为1:10,000.
正确应用WWS可以检测多种NPEV类型.
直接,分子和基于序列的测试正在推进WWS的能力.

结论:

废水监测 (WWS) 是一种廉价,灵活和可靠的基于人口的工具.
它有助于监测疫情和病毒病原体的出现/重新出现.
卫星卫星补充了基于案例的监测,以全面监测公共卫生.

关键词:

环境监督环境监督环境监督环境监督环境监督非脊髓灰质炎肠道病毒脊髓灰质炎病毒是什么?废水监管监督废水的情况.

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