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开发一种多重PCR试验,以有效地检测两种潜在的益生菌菌株,使用基于全基因组的原始剂

Despoina E Kiousi¹, Dimitrios M Karadedos¹, Anastasia Sykoudi¹

¹Department of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece.

|October 28, 2023

概括

一种新的多重PCR方法可以准确检测特定的益生菌菌株,Lactiplantibacillus plantarum L125和Lp. pentosus L33,在食品中使用. 这种菌株特定的检测对于功能性食品至关重要,并且可以适应其他细菌菌株.

科学领域:

微生物学微生物学
食品科学食品科学食品科学
分子生物学分子生物学

背景情况:

益生菌提供菌株特定的健康益处,但在食物中难以检测.
目前的检测方法缺乏足够的菌株级别歧视.
准确的识别对于功能性食品的质量和有效性至关重要.

研究的目的:

开发一种高度特定的方法来检测两种益生菌乳酸细菌 (LAB) 菌株.
为了能够可靠地识别Lactiplantibacillus plantarum L125和Lp. 在食品矩阵中的 pentosus L33.
创建一种用于检测细菌菌株的多功能工具.

主要方法:

进行比较基因组分析,以确定目标菌株的独特基因组区域.
设计和选择用于多重PCR的特定菌株原料.
在单种植和复杂食品样本 (酸奶) 中验证试验.

主要成果:

成功开发了一种多重PCR试验.
该测试为每个目标菌株生成了不同的DNA带纹模式.
该方法证明了在酸奶样本中检测益生菌LAB的适用性.

结论:

开发的多重PCR试验为益生菌LAB提供了高菌株特异性检测.
这种方法提供了一个生物技术上适用的解决方案,用于功能性食品的质量控制.
该方法可以适应用于使用全基因组测序数据检测其他细菌菌株.

关键词:

细菌的鉴定细菌的鉴定进行比较的基因组学.乳酸细菌是一种乳酸细菌.多重复合PCR是一种复合PCR.益生菌是一种益生菌.一个特定菌株的特定菌株.整个基因组的测序.

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