黑色NDM-1阳性Acinetobacter baumannii在希腊中部被隔离的分子表征
Katerina Tsilipounidaki1, Christos-George Gkountinoudis1, Zoi Florou1
1Faculty of Medicine, University of Thessaly, 41500 Larissa, Greece.
Microorganisms
|October 28, 2023
概括
这项研究报告了在希腊首次出现多个NDM-1阳性Acinetobacter baumannii克隆. 分子表征揭示了blaNDM-1基因的多样化遗传环境,突出显示了监测的必要性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 抗菌素耐药性 (AMR) 的上升构成了全球健康的重大威胁.
- 耐卡巴胺的Acinetobacter baumannii (CRAB) 是一个关键的病原体,通常与医院获得的感染有关.
- 新德里金属β-乳酸酶 (NDM-1) 酶赋予了对广泛的β-乳酸抗生素的耐药性,包括卡巴.
研究的目的:
- 检测和分子表征NDM-1阳性Acinetobacter baumannii从希腊的一家三级护理医院的分离物.
- 研究这些产生NDM-1菌株的遗传多样性,抗体和毒性因素.
- 了解blaNDM-1基因的基因环境及其水平基因转移潜力.
主要方法:
- 全基因组测序 (WGS) 用于全面的分子分析.
- 进行了巴斯图尔多位点测序类型 (MLST) 测定,以确定分离物的克隆系.
- 对WGS数据的分析确定了NDM-1基因的遗传上下文,包括周围的移动遗传元素,并描述了完整的抗体和毒性基因含量.
主要成果:
- 在2022年12月至2023年8月期间,发现了9个NDM-1阳性Acinetobacter baumannii分离体.
- 这些分离物属于四种不同的序列类型 (STs 160,2,85,和2493),表明多克隆的出现.
- 观察到NDM-1基因的两个不同的遗传环境:ST160中的Tn125和STs 2,85和2493中的Tn7382.
- 所有分离物体都携带了额外的耐药基因,从而产生了对多种类型抗微生物药物的耐药性.
结论:
- 这项研究代表了在希腊首次报告多克隆NDM-1阳性Acinetobacter baumannii的第一份报告.
- 这些发现突显了NDM-1成功建立和扩散,产生A. 在医疗机构内跨越不同克隆.
- 严格的监测协议对于监测和限制NDM-1基因在希腊境内及境外进一步传播至关重要.
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