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夜 primrose 提取物调节TYMS表达通过SP1转录因子在恶性腹半瘤中的表达

Małgorzata Chmielewska-Kassassir¹, Katarzyna Sobierajska², Wojciech M Ciszewski²

¹Department of Structural Biology, Medical University of Lodz, Żeligowskiego 7/9, 90-752 Lodz, Poland.

|October 28, 2023

概括

欧帕叶 (EPE) 和其成分,酸和酸,通过与TYMS促进体上的SP1动机结合来降低基酸合成酶 (TYMS) 的下调,从而逆转癌细胞的侵入性.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

恶性多层层层瘤 (MPM) 是一种具有攻击性的癌症.
乙基酸合成酶 (TYMS) 是DNA合成中的一个关键酶,也是癌症治疗的目标.
皮质到介质细胞的过渡 (EMT) 对于癌症的入侵和转移至关重要.

研究的目的:

阐明Eupatorium perfoliatum (EPE) 在MPM中降低TYMS表达的机制.
调查SP1转录因子在EPE对TYMS影响中的作用.

主要方法:

在MPM患者数据中分析TYMS mRNA和EMT生物标志物 (GSE51024).
在实验室研究中,使用使用EPE治疗的侵入性MPM细胞系.
使用生物信息学和分子分析 (电泳运动移位,双露西法酶记者分析) 识别TYMS促进体上的SP1结合位.
染色体免疫沉 (ChIP) 和Re-ChIP用于评估蛋白质-DNA相互作用.

主要成果:

过度表达的TYMS与MPM患者的EMT标志物 (TWIST1,FN1,N-cadherin) 有积极的相关性.
EPE及其成分 (酸,酸) 降低了侵入性MPM细胞中的TYMS表达.
在TYMS促进体上,EPE与特定的SP1动机相互作用,通过光酶试验证实了这一点.
EPE治疗影响了SP1的核转位.

结论:

EPE组件通过占据TYMS促进体上的SP1结合位来抑制TYMS表达.
这种机制逆转了侵入性MPM细胞中的EMT表型.
需要进一步研究EPE的多化合物与SP1的分子对接.

关键词:

表皮细胞到介质细胞的过渡.晚上灵花提取物晚上灵花提取物恶性多层层层层瘤.特异性蛋白质 1 核因子提米基酸合成酶是什么意思

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