患有早期肠的患者的粘膜微生物组:从短读和长读16SrRNA测序的推断
Zoe Welham1, Jun Li1, Alexander F Engel2,3
1Bowel Cancer and Biomarker Laboratory, School of Medical Sciences, The University of Sydney, Sydney 2065, Australia.
Cancers
|October 28, 2023
概括
与没有多体的患者相比,肠道多体患者表现出改变的肠道细菌,特别是增加的Ruminococcus gnavus. 测序技术对研究结果产生影响,突出显示R. gnavus是早期结直肠瘤的一致标志物.
科学领域:
- 微生物组研究的研究.
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
背景情况:
- 结肠直肠癌 (CRC) 与肠道失生症有关,但癌前多体中的粘膜微生物群不太了解.
- 短读16SrRNA测序在微生物组研究中产生了不一致的结果.
- 研究粘膜微生物组对于了解结直肠瘤早期阶段至关重要.
研究的目的:
- 为了比较有或没有肠的患者的粘膜微生物群.
- 评估短读与长读16S rRNA测序对发现的影响.
- 为了识别与肠道多相关的特定细菌种群.
主要方法:
- 在结肠镜检查期间从多患者和对照组收集的粘膜样本.
- 使用了短读和PacBio长读16SrRNA测序.
- 分析了微生物多样性和安普利康序列变异 (ASV) 的差异丰度.
主要成果:
- 两组之间没有发现总体微生物多样性的显著差异.
- 在多患者中观察到高的Ruminococcus gnavus和Escherichia coli的ASVs.
- 在多患者中发现了降低的Parabacteroides merdae,Veillonella nakazawae和Sutterella wadsworthensis的ASVs.
- 鲁米诺科克斯 (Ruminococcus gnavus) 是唯一一种在两种测序方法中一致改变的细菌.
结论:
- 肠与粘膜微生物组的特定变化有关,而不仅仅是整体多样性变化.
- 鲁米诺科克斯 (Ruminococcus gnavus) 可能作为早期肠道瘤的潜在生物标志物.
- 测序技术和生物信息学的差异可能会影响研究结果.
- 观察到的细菌变化表明在早期结直肠瘤中存在炎症性微环境.
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