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Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
Published on: June 26, 2019
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Slc11 Synapomorphy:一个保存的3D框架,可以连接载体形状交换机
1Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Laval, QC H7V 1B7, Canada.
International journal of molecular sciences
|October 28, 2023
概括
跨膜载体Slc11家族使用质子梯度来导入重要金属离子,如和铁. 关键的进化部位 (synapomorphy) 控制金属的选择性和运输,即使在快速进化的细菌病原体中也是如此.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- Slc11家族的跨膜载体对于进口必需的双价金属离子 (Me2+),如和铁,利用质子动力至关重要.
- 在 prokaryotes (MntH) 和eukaryotes (Nramp) 中保存,Slc11载体表现出高亲缘关系的Me2+进口,与质子合运输机制的重复进化.
- 细菌获得Nramp基因表明适应性进化有利于机会性病原体.
研究的目的:
- 阐明Slc11家族跨膜载体的进化机制和结构基础.
- 为了研究特定的进化保存点 (synapomorphy) 在金属离子选择性和传输中的作用.
- 为了比较不同细菌群 (MCb和MCg) 和它们的真核祖先之间的载体动力学和可塑性.
主要方法:
- 使用AlphaFold (AF2) /Colabfold (CF) 来进行细菌Slc11序列的3D结构预测.
- 在基基因突变中使用,准假设的synapomorphic网站和联网的残留社区.
- 将原生和突变结构模型进行比较,以分析形状变化 (外向开放到内向开放状态) 和门机制.
主要成果:
- AF2/CF模型揭示了MCb基团的中间体,在开放和封闭状态之间过渡,由synapomorphic网站驱动.
- 确定Slc11的协同形态是Me2+-选择性构造切换的关键决定因素,控制内外门.
- 虽然MCg1异种表现出明显的结构可塑性和改变的质子网络,但同型位点也调节了它们的门机制,尽管有类特异性的表观相互作用.
结论:
- Slc11 协同形态形成了一个保存的 3D 网络,对于跨膜载体中的金属选择性形状转换至关重要.
- 像MCg1这样的分类中的不同的进化途径,特别是像*Bordetella*和*Achromobacter*这样的属,通过表观相互作用影响载体形状和动态.
- 选择性运输的核心机制Me2+在不断变化的群体中保持不变,适应各种环境压力.
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