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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Plant Tissue Culture

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Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
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相关实验视频

Updated: Jul 12, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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替代拼接变化:在作物改进计划中获取和利用作物改进方案

Sangam L Dwivedi1, Luis Felipe Quiroz2, Anireddy S N Reddy3

  • 1Independent Researcher, Hyderabad 500016, India.

International journal of molecular sciences
|October 28, 2023
PubMed
概括
此摘要是机器生成的。

替代拼接 (AS) 从单个基因生成多种不同的信使RNA (mRNA),影响植物特征. 了解AS变异是提高作物弹性和生产率的关键.

关键词:
替代性拼接是一种替代性的拼接.生物节奏是生物节奏.化和多化是化和多化.基因开采是指基因开采的过程.异质化异质化是什么意思营养物质的恒常性是营养的恒常性.植物现象学和建筑学这种共生体是共生体.转录组和蛋白质组多样性的多样性

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科学领域:

  • 植物分子生物学 植物分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 替代拼接 (AS) 是一种基本的基因调节机制,存在于真核生物,包括植物.
  • AS从单个前体mRNA (pre-mRNA) 产生多个信使RNA (mRNA) 转录,从而增加转录组和蛋白质组的多样性.
  • 这个过程在调节植物生长,发育,繁殖和应激反应的各个方面发挥着至关重要的作用.

研究的目的:

  • 审查植物中替代拼接 (AS) 变化的意义.
  • 探索AS如何促进表型多样性和农业学上有益的特征.
  • 在育种计划中突出AS在作物改进方面的潜力.

主要方法:

  • 利用下一代测序和奥米克技术的进步.
  • 利用生物信息学工具和计算方法进行AS量化和可视化.
  • 与AS变异一起分析基因表达.

主要成果:

  • AS在植物中普遍存在,并产生了相当大的转录基因和蛋白质基因多样性.
  • AS变体与生长,发育,繁殖和应激耐受性的定量性质变化有关.
  • 化,多化和环境变化可以推动与理想特征相关的新型AS变体的演变.
  • AS有助于复杂的现象,如异构和植物共生.

结论:

  • 编目和利用AS变异为增强主食作物提供了一个有希望的途径.
  • 可以利用AS变化来提高气候弹性,资源利用效率,生产力和营养质量.
  • 了解AS提供了核心时钟基因和环境线索之间的机械联系,这对于适应性繁殖至关重要.