在Elsholtzieae中的等质体的比较分析:植物遗传关系和潜在的分子标记物
Xiong-De Tu1,2, Zhuang Zhao2, Cheng-Yuan Zhou2
1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
International journal of molecular sciences
|October 28, 2023
概括
这项研究对两个Mosla塑体进行了测序,揭示了Elsholtzie.ae.中保存的基因含量和结构. 在这个有价值的植物部落中,可变区域被确定为DNA条形码和遗传学分析的分子标记.
科学领域:
- 植物学 植物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 埃尔肖尔茨科 (Lamiaceae家族) 部落具有重要的药用,芳香,和装饰价值.
- 尽管它很重要,但在Elsholtzieae.ae.中,有限的分子标记物和塑体可用于遗传学研究.
- 高通量测序提供了一种强大的方法,可以为这个研究不足的群体生成基因组资源.
研究的目的:
- 组装和描述两个莫斯拉物种 (M. dianthera和M. scabra) 的完整塑体.
- 将这些塑体与其他可用的Elsholtzieae塑体进行比较,以获得遗传学见解.
- 在Elsholtzieae部落中识别潜在的分子标记物用于分类学和DNA条形码应用.
主要方法:
- 使用高通量测序,获得了莫斯拉·迪安特拉和莫斯拉·斯卡布拉的塑体序列.
- 质体结构,基因含量 (蛋白质编码基因,tRNA,rRNA,伪基因) 和大小被分析并与Elsholtzieae物种进行了比较.
- 对比基因组分析确定了高变异性的区域,包括编码序列 (CDS) 和基因间区域 (IGS).
- 用CDS和CDS+IGS组合数据集进行了家族遗传学分析.
主要成果:
- 成功组装了莫斯拉物种的两个完整的塑体体,展示了植物塑体体的典型四部分结构.
- 埃尔肖尔特兹叶群的塑体从148,288到152,602 bp不等,含有132个基因 (113个是独一无二的),观察到微小的变异.
- 值得注意的变异包括Elsholtzia densa缺少rps19伪基,以及E. densa.修改的IR边界.
- 在E. eriostchya plastid基因组的特定区域观察到GC含量增加.
- 可变CDS和IGS序列被确定为有前途的分子标记物.
- 遗传学分析解决了四个单一的属,莫斯拉和佩里拉形成了哥林索尼亚的姐妹类,埃尔肖尔茨亚是外群.
结论:
- 这项研究为Mosla属提供了第一个塑体序列,丰富了Elsholtzieae的基因组资源.
- 识别的变量区域为物种识别,DNA条形码以及解决Elsholtzieae.ae.内部的家族遗传关系提供了有价值的分子标记.
- 遗传学分析澄清了各属之间的进化关系,支持关键群体的单,以及莫斯拉和佩里拉的位置.
- 这些发现有助于更好地了解Elsholtzieae部落,促进进一步的研究和利用其宝贵的资源.
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