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患有非酒精性脂肪性肝病患者的肠道微生物群模式:使用三种分析方法进行全面评估

Anna V Korobeinikova¹, Olga A Zlobovskaya¹, Anna F Sheptulina²

¹Centre for Strategic Planning and Management of Biomedical Health Risks of Federal Medical Biological Agency, Pogodinskaya Str., 10/1, 119121 Moscow, Russia.

International journal of molecular sciences

|October 28, 2023

概括

非酒精性脂肪性肝病 (NAFLD) 与显著的肠道微生物组变化有关. 这项研究确定了NAFLD患者的特定细菌变化,突出了肠道微生物群.

科学领域:

微生物学微生物学
胃肠病学胃肠病学
代谢疾病代谢疾病

背景情况:

非酒精性脂肪肝 (NAFLD) 是一个普遍的全球健康问题,影响大约25%的成年人.
越来越多的证据表明,肠道微生物群失生症与NAFLD的发病和进展有关.
对复杂的肠道微生物组的全面分析对于了解NAFLD至关重要.

研究的目的:

为了研究和比较NAFLD患者没有显著纤维化与健康对照的肠道微生物组成.
用多种方法对微生物差异进行彻底评估.
为了确定与NAFLD相关的特定微生物种群.

主要方法:

采用了结合MALDI-TOF-MS,qPCR和16S rRNA基因下一代测序 (NGS) 的多omics方法.
利用多种统计方法分析微生物社区结构和差异.
在NAFLD患者和对照组之间比较肠道微生物群概况.

主要成果:

在NAFLD患者中确定了独特的微生物特征,包括在Lachnospiraceae (Dorea sp.) 中进行丰富. 和 Veillonellaceae. 这种类型的植物.
在Bifidobacteriaceae,Lactobacillaceae和Enterococcaceae (Enterococcus faecium,Enterococcus faecalis) 等特定家族中观察到的变化.
检测到特定方法的变化,如增加的Candida krusei和减少的Bacteroides uniformis (MALDI-TOF-MS),以及增加的Fusobacterium核酸菌 (qPCR).

结论:

NAFLD与肠道微生物群组成的变化有显著的关联.
多种分析技术证实了NAFLD中不同的微生物特征.
需要进一步的研究来确定因果关系和微生物代谢物在NAFLD中的作用.

关键词:

16S rRNA 是一个16S rRNA.的细菌培养.我们的肠道微生物组.甲基基因组 (metagenome) 是一个基因组.非酒精性脂肪性肝病是非酒精性脂肪性肝病.qPCRR 是一个很好的方法.

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