使用人类肠道病毒中发现的替代遗传密码的新型菌体组的基因组
Igor Babkin1,2, Artem Tikunov1,2, Vera Morozova1
1Federal State Public Scientific Institution «Institute of Chemical Biology and Fundamental Medicine», Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
International journal of molecular sciences
|October 28, 2023
概括
在人类肠道病毒组中发现了一种具有异常遗传密码的新型菌体基因组 (phAss-1). 这一发现强调了在菌体基因组分析和数据沉积中需要多样化的翻译表.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
背景情况:
- 甲基因组学使得在微生物群落中能够检测菌体基因组.
- 菌体中的替代遗传代码阻碍了精确的基因组分析.
- 人的肠道病毒组是微生物多样性的丰富来源.
研究的目的:
- 报告发现和描述一种具有不寻常遗传密码的新型菌体基因组.
- 调查替代遗传代码对菌体基因组分析的影响.
- 根据基因组相似性建立一个新的菌体属.
主要方法:
- 人体肠道病毒基因组数据的新组合.
- 对已识别的菌体序列进行基因组分析.
- 对比基因组学和遗传学分析.
- 抑制器tRNA基因和非正规的停止编码子的识别.
主要成果:
- 在人类肠道病毒组中发现了不寻常的菌体基因组phAss-1 (135,976 bp).
- 在phAss-1中识别了41个ORF与TAG停止编码子和3个抑制器tRNA基因.
- 在元基因组数据中检测出两个相似的菌体基因组 (BK046881_ctckW2,BK025033_ct6IQ4).
- 基因组和遗传学分析支持了一个新属的提议, *Phassvirus*.
结论:
- 发现phAss-1和相关菌体需要使用替代翻译表来进行准确的菌体基因组分析.
- 拟议的*Phassvirus*属代表着具有独特遗传特征的菌体的一个独特的血统.
- 准确的菌体基因组沉积在数据库中需要考虑不同的翻译系统.
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