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来自葡萄牙的Mycobacterium abscessus复杂分离体的基因组规模表征

Sofia Carneiro^1,2, Miguel Pinto³, Sónia Silva¹

¹National Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, Portugal.

International journal of molecular sciences

|October 28, 2023

概括

葡萄牙的Mycobacterium abscessus复合体 (MABC) 显示出高度的遗传多样性和多药性耐药性. 这项研究是迈向该国MABC基因组监测系统的第一步.

科学领域:

临床微生物学临床微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

菌根综合体 (MABC) 是一个日益严重的全球健康问题,导致难以治疗的,多药耐药的感染.
主导循环的MABC克隆 (DCCs) 与增加的流行率,抗菌素耐药性和较差的患者结局有关.
对于葡萄牙传播的MABC菌株,基因组数据有限.

研究的目的:

调查葡萄牙MABC菌株的遗传多样性.
描述这些MABC菌株的抗菌素耐药性概况.
为葡萄牙的MABC基因组监测系统奠定基础.

主要方法:

全基因组多位序列类型化 (wgMLST) 用于基因多样性评估和亚种差异化.
分离物被分类为主要的循环克隆 (DCC).
使用表型,分子和基因组方法评估了抗菌素耐药性.

主要成果:

分析了2014年至2022年在葡萄牙分离的30种MABC菌株.
大多数隔离物对至少两种抗微生物药物表现出耐药性,观察到表型和基因型耐药性数据之间的差异.
葡萄牙MABC基因组显示出显著的多样性,表明某些血统的潜在国际或跨境传播.

结论:

这项研究为葡萄牙流传的MABC的遗传构成和抗菌素耐药性模式提供了关键的见解.
这些发现突显了MABC血统在国际上传播的潜力.
这项研究代表了在葡萄牙国家卫生研究院 (NIH) 建立MABC基因组监测系统的基本步骤.

关键词:

在MABCBC中,MABC是最重要的.这种细菌是Mycobacterium abscessus.葡萄牙葡萄牙葡萄牙抗微生物耐药性抗微生物耐药性基因组多样性基因组多样性没有结核的真菌菌.wgMLSTST 在线观看进行全基因组测序.

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