抑制GhTUBB1可降低Gossypium hirsutum中的植物高度
Lihua Zhang1,2, Caixia Ma1,3, Lihua Wang1
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
International journal of molecular sciences
|October 28, 2023
概括
在棉花中引入Ammopiptanthus mongolicus C-重复结合因子1 (AmCBF1) 导致矮化. AmCBF1 直接抑制了 Gossypium hirsutum Tubulin beta-1 (GhTUBB1) 的表达,为繁殖紧品种提供了一个新的目标.
科学领域:
- 植物遗传学和育种.
- 分子生物学分子生物学
- 农作物科学 农作物科学
背景情况:
- 绿色革命的矮品种往往有不良的副作用,原因是 gibberellin 缺乏.
- 开发新的矮人基因型对于改善作物育种至关重要.
- 在之前的研究中,在引入Ammopiptanthus mongolicus C-重复结合因子1 (AmCBF1) 后,发现了矮棉线 (L28,L30) 与下调的Gossypium hirsutum Tubulin beta-1 (GhTUBB1) 结合.
研究的目的:
- 调查 GhTUBB1 在棉花矮化中的作用.
- 确定AmCBF1和GhTUBB1.1之间的监管关系.
- 为了确定繁殖紧棉花品种的潜在新目标.
主要方法:
- 在棉花中抑制 GhTUBB1 的基因沉默.
- 酵母的一种混合测试.
- 双露西法酶记者测定.
主要成果:
- 在棉花中杀GhTUBB1导致了矮人表型.
- 抑制AmCBF1在矮线中部分恢复了GhTUBB1的表达.
- AmCBF1直接与GhTUBB1促进体中的CRT/DRE元素结合,对其表达进行负调节.
结论:
- GhTUBB1 是一个关键基因,在棉花系L28和L30中为矮人表型作出贡献.
- AmCBF1 负面调节 GhTUBB1 的表达,可能是通过直接促进体结合.
- GhTUBB1代表了一个有前途的新目标,用于开发矮种和紧种的棉花.
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