益生菌素对大脑细胞功能和血脑屏障透性的影响
Hadi Younes1,2,3, Ioanna Kyritsi1,2,3, Zineb Mahrougui1,2,3
1University Grenoble-Alpes, CEDEX 9, 38043 Grenoble, France.
International journal of molecular sciences
|October 28, 2023
概括
催生素 (PROKs) 不同地影响大脑细胞的增殖和迁移. 这些蛋白质增加了血脑屏障 (BBB) 的透性,但抗体可以阻止这种效应.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 促生素 (PROKs) 是信号蛋白,在各种组织中具有已知的作用.
- 它们的特定功能和对不同类型的大脑细胞和血脑屏障 (BBB) 完整性的影响尚未完全理解.
研究的目的:
- 研究PROK1和PROK2对小鼠大脑微血管内皮细胞 (bEnd.3),星球细胞 (C8.D30) 和神经元 (N2a) 的影响.
- 确定PROKs对BBB共同培养模型和受体介导信号的影响.
主要方法:
- 利用西方斑块分析细胞系跨细胞因子受体 (PROKR1和PROKR2) 的表达.
- 采用时间间隔显微镜来评估细胞增殖和迁移.
- 使用已建立的共同培养模型和受体对抗剂 (PROKR1的PC7和PROKR2的PKR-A) 调查了BBB的透性.
主要成果:
- PROK1降低了神经细胞的增殖,但并没有降低内皮细胞或天体细胞的增殖.
- 在所有测试的细胞系中,PROK2降低了增殖.
- 无论是PROK1还是PROK2,都增强了细胞迁移.
- PROK2诱导的天体细胞迁移被PROKR抗剂抑制.
- PROKs增加了BBB的透性,这是PROKR抗体可以逆转的效果.
结论:
- PROK1和PROK2对大脑细胞的增殖和迁移具有明显的和重叠的影响.
- 在调节BBB透性方面,PROKs起着重要的作用.
- 准PROK受体可能为涉及BBB功能障碍的疾病提供治疗策略.
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