PagMYB205 通过反应性氧物种扫除和根活力调节对树盐分耐受性产生负面影响
Lieding Zhou1, Xuhui Huan1, Kai Zhao1
1College of Forestry, Shanxi Agricultural University, Jinzhong 030801, China.
International journal of molecular sciences
|October 28, 2023
概括
豆的 PagMYB205 基因对盐耐受性产生负面影响. 抑制其表达 (RNAi) 改善了根源活力和抗氧化酶活性,增强了在盐应激下的生存能力. 过度表达 (OX) 恶化了这些影响.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 无生物性压力生理学生理学
背景情况:
- 盐应激显著阻碍了植物的生长和发育.
- MYB转录因子对于植物发育和应激反应至关重要.
研究的目的:
- 为了研究树PagMYB205基因在盐应激耐受性中的作用.
- 分析PagMYB205过度表达和抑制对树生长和对盐的生理反应的影响.
主要方法:
- 在 (Populus abla × Populus glandulosa) 中克隆和功能分析PagMYB205基因.
- 产生转基因木线与PagMYB205过度表达 (OX) 和RNA干扰 (RNAi).
- 在盐应激下对形态和生理参数的评估,包括根活力,甲酸 (MDA) 含量和抗氧化酶活性 (POD,SOD,CAT).
主要成果:
- PagMYB205编码了一个核局部蛋白质,没有自我激活活性.
- 在盐应激下,RNAi线表现出更高的植根率和根活力,与野生类型 (WT) 和OX线相比,MDA含量较低.
- 通过增加POD,SOD和CAT酶活动,RNAi系表现出增强的活性氧物种 (ROS) 清理能力.
- 具有最低 PagMYB205 表达的 RNAi3 线显示出最佳的盐耐受性.
结论:
- 转录因子PagMYB205负面调节树的盐应激耐受性.
- PagMYB205通过调节抗氧化酶活性和根源活力来影响盐分耐受性.
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