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全基因组DNA甲基化和转录组学的整合确定了潜在的基因,这些基因可以调节的骨肌肉的发育

Yinglin Lu¹, Jing Zhou¹, Fan Li¹

¹Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

International journal of molecular sciences

|October 28, 2023

概括

这项研究揭示了胚胎骨肌肉发育中的关键DNA甲基化调节剂. 我们确定了差异甲基化的基因和途径,为肌肉生长的表观遗传控制提供了洞察力.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发展生物学发展生物学
基因组学就是基因组学.

背景情况:

基因甲基化对于骨肌肉发育至关重要.
胚胎骨肌中的特定表观遗传调节剂在很大程度上是未知的.

研究的目的:

为了确定DNA甲基化模式和调节器在胚胎骨肌肉发育.
探索肌肉发育期间DNA甲基化和基因表达之间的关系.

主要方法:

全基因组双硫酸盐测序 (WGBS) 和转录组测序在胚胎骨肌上在E21和E28进行.
用WGBS和转录学数据的综合分析来识别差异甲基化区域 (DMR) 和差异甲基化基因 (DMG).
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.

主要成果:

基因组甲基化主要发生在CG环境中,在内子,外子和促进子中含量很高.
确定了7902个DMR和3174个DMG,其中1072个DMG与差异表达基因 (DEG) 有负相关.
关键的丰富途径包括MAPK信号传递,Wnt信号传递和胰岛素信号传递. 确定了像*Idh3a*,*Got1*和*Klf2*这样的特定基因可能被DNA甲基化调节.

结论:

这项研究阐明了DNA甲基化在胚胎骨肌肉发育中的作用.
已识别的DMG和相关的信号通路为肌肉形成的表观遗传调节提供了新的见解.
基因促进器中的CpG岛屿可能在调节骨肌肉发育方面发挥关键作用.

关键词:

通过DNA甲基化.在RNA-seqqq.这是WGBS的WGBS.胚胎子的子骨肌肉的发展

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