对斑点刀 (Oplegnathus punctatus) 中重复的对应物进行的比较研究显示了功能多样化
Xinxin Du1,2, Haiyang Yu1,2, Yujue Wang2
1School of Life Science and Bioengineering, Jining University, Jining 273155, China.
Genes
|October 28, 2023
概括
两种分叉盒L2 (foxl2) 对应物,foxl2和foxl2l,可能起源于斑点刀中的早期全基因组复制 (WGD). 这些基因表现出明显的表达模式,并证实了 gonadal 发育中的功能分歧.
科学领域:
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
- 生殖生物学 生殖生物学
背景情况:
- 叉盒L基因家族,包括foxl2,对于生殖腺发育和生殖调节至关重要.
- 整个基因组重复 (WGD) 事件显著影响了脊椎动物的进化,但远程生物中的foxl2重复仍未得到充分研究.
研究的目的:
- 为了识别和描述斑点刀 (Oplegnathus punctatus) 中的狐类型.
- 为了研究这些类比的进化起源和功能分歧.
主要方法:
- 转录组分析以确定foxl2的相对应物.
- 遗传学重建,合成分析和分子进化试验.
- 定量实时PCR (qRT-PCR) 和现场杂交 (ISH) 用于表达模式分析.
- 动机扫描分析以预测功能分歧.
主要成果:
- 在斑点的刀中发现了两个foxl2对应物,foxl2和foxl2l.
- 在卵巢中,foxl2的表达更高,而在丸中,foxl2的表达更高.
- 遗传学和合成分析表明起源于前两个WGD事件.
- 观察到有证据表明foxl2和foxl2l之间的功能差异.
结论:
- 斑点刀中的foxl2和foxl2l很可能是从早期的WGD中产生的.
- 这些对应物在转录上是活跃的,并表现出功能分歧的迹象.
- 需要进一步的研究才能充分阐明这些重复基因的功能.
相关概念视频
Gene Duplication and Divergence
6.1K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.1K
Gene Families
8.8K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
8.8K
Genetics of Speciation
19.3K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
19.3K
Convergent Evolution
27.8K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
27.8K
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
Multi-species Conserved Sequences
3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K


