相关实验视频

Updated: Jul 12, 2025

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

通过在各种人体组织和细胞系中通过长读序列测序评估长间分散元素 (LINE) 的表达

Karleena Rybacki^1,2, Mingyi Xia^1,2, Mian Umair Ahsan²

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

|October 28, 2023

概括

这项研究开发了一个生物信息学管道来分析全长的长间分散元素 (LINE) 转录. 活性LINE-1 (L1) 元素在癌细胞中更为丰富,揭示了它们的基因组景观.

科学领域:

基因组学和生物信息学
分子生物学分子生物学
癌症研究癌症研究

背景情况:

可转移元素,包括长间散元素 (LINEs),是可改变基因组结构的移动DNA序列.
LINE活动有助于人类群体的基因组变异和癌症的体质变化.
长读RNA测序提供了一种研究全长LINE转录的方法.

研究的目的:

开发一种新的生物信息管道,用于识别和量化活跃的,全长的LINE转录.
分析LINE-1 (L1) 表达在各种人体组织和细胞系中的情况.
为了比较癌细胞和正常细胞系之间的L1表达模式.

主要方法:

使用 RepeatMasker 来识别从长读转录组数据中的 LINE-1 (L1) 转录.
实施过标准,包括转录开始位置,分歧和长度,以提高准确性.
应用生物信息学分析从人类组织和细胞系中长时间读取的RNA测序数据.

主要成果:

成功开发并应用了用于识别和量化全长LINE转录的管道.
与正常细胞系相比,在癌细胞系中观察到年轻LINE的高表达.
在癌症样本中发现完整的L1位点的表达特别高.

结论:

开发的生物信息学管道使得全长LINE转录的全面分析成为可能.
在癌症中,LINE-1表达显著改变,年轻LINE和完整的L1位点的活性增加.
这项研究提供了对人类组织和细胞系L1表达格局的洞察.

关键词:

线路-1 线路-1 在线长时间散的元素.在这里,我们可以看到TTETETE.基因表达的基因表达方式长时间阅读序列排序.可转移的元素可以转移.

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Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Published on: July 27, 2019

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

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