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在多重黑草 (Alopecurus myosuroides) 种群中的染色体外圆形DNA含量的序列表征

Wangfang Fu¹, Dana R MacGregor², David Comont²

¹Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634, USA.

|October 28, 2023

概括

黑草杂草具有含有除草剂排毒基因的额外染色体圆形DNA (eccDNA). 这些eccDNA可能会在抗除草剂 (HR) 种群中快速适应.

科学领域:

植物遗传学植物遗传学
杂草生物学杂草生物学
分子进化的分子进化.

背景情况:

黑草 (Alopecurus myosuroides) 是西欧冬季小麦的主要杂草.
黑草中广泛的多种除草剂耐药性有助于其成功.
之前的研究表明,非位抗性 (NTSR) 的多基因遗传来自常态遗传变异.

研究的目的:

为了研究额外染色体圆形DNA (eccDNA) 在黑草中抗除草剂的作用.
识别和比较抗除草剂 (HR) 和抗除草剂 (HS) 黑草种群中eccDNAs的编码内容.
将eccDNA含量映射到黑草基因组和已知的定量特征位置 (QTL) 进行除草剂耐药性.

主要方法:

利用CIDER-seq管道检测和分析HR和HS黑草植物及其父种群中的eccDNA.
识别了在耐药和敏感种群内和之间编码结构,预测的基因和功能蛋白质领域的相似性和差异.
将eccDNA序列映射到A. myosuroides参考基因组和已知的抗除草剂的QTL区域.

主要成果:

HR和HS黑草种群都携带了eccDNA.
在eccDNA中含有参与除草剂排毒的基因,包括P450细胞染色体,ATP结合盒载体和谷氨转移酶 (例如AmGSTF1).
在染色体1,5和7的特定区域,eccDNA基因密度很高.eccDNA序列与已知的15个抗除草药QTL区域中的12个相匹配.

结论:

黑草具有具有染色体基因同类的eccDNA,类似于其他杂草.
这些eccDNA可能有助于遗传异质性,并提供了一个快速适应除草剂治疗等压力的机制.
在面临除草剂选择压力的杂草种群中,eccDNA提供了进化创新的潜在来源.

关键词:

黑草是一种黑草.这就是为什么eccDNADNA.抗除草剂耐药性抗除草剂耐药性快速适应快速适应

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