在Bemisia tabaci中在不同温度应力下可访问的染色素景观和转录组的特征
Xiaona Shen1,2, Xiaodi Wang1, Nianwan Yang1,3
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Genes
|October 28, 2023
概括
烟草适应温度压力,通过改变色素的可访问性. 这种表观遗传机制调节基因表达,影响害虫的生存,并提供对分子反应的见解.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 烟草 (B. tabaci) 是一种具有显著温度耐受性的全球侵入性害虫.
- 在B. tabaci种群中,温度耐受性各不相同,这表明了适应机制.
- 在B. tabaci中已知表观遗传调节,暗示其在热适应中的作用.
研究的目的:
- 研究B. tabaci.在热应激下对染色质可访问性和全基因组转录组的变化.
- 阐明表观遗传机制在B. tabaci的温度耐受性中的作用.
主要方法:
- 用高通量测序 (ATAC-seq) 检测转化酶可访问的染色质,以绘制染色质可访问性的地图.
- RNA测序 (RNA-seq) 来分析全基因组的转录组.
- 整合ATAC-seq和RNA-seq数据以将基因表达与染色质可访问性相关联.
主要成果:
- 基因的转录活性与B. tabaci.中的染色质可访问性有很强的相关性.
- 高温压力与转录激活的基因表达和低温抵抗基因的抑制有关.
- 低温抗压力包括调节酶活动,而高温抗压力包括调节新陈代谢和有机物质合成,这两者均由染色质可访问性变化介导.
结论:
- 通过染色质可访问性的表观遗传适应在B. tabaci对温度压力的反应中起着至关重要的作用.
- 这项研究为了解入侵物种中基因表达调节和分子对热应激反应的反应提供了基础.
- 改变的染色质可访问性为B. tabaci在不同温度下生存提供了一个新的调节机制.
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