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调节失调的微RNA表达与桃体癌-A试点研究中的TERT促进器突变相关

Mi Jung Kwon¹, Ha Young Park², Joong Seob Lee³

¹Department of Pathology, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang 14068, Republic of Korea.

Life (Basel, Switzerland)

|October 28, 2023

概括

人类乳头瘤病毒 (HPV) 阳性桃体状细胞癌显示出不同的微RNA (miRNA) 表达特征. 像hsa-miR-1285-5p和hsa-miR-663a这样的特定miRNA可以作为早期诊断和潜在治疗点的生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
病毒学病毒学

背景情况:

状状细胞癌 (TSCCs) 经常对人类乳头瘤病毒 (HPV) 呈阳性.
微RNA (miRNA) 表达在TSCC中的作用,特别是关于HPV状态和遗传突变的作用,仍未得到充分研究.
以前的研究集中在临床病理学和遗传数据上,需要对miRNA配置文件进行更深入的调查.

研究的目的:

在TSCC组织中分析miRNA表达与HPV状况相关的情况.
确定TSCC潜在的诊断生物标志物和治疗点.
为了研究miRNA表达,HPV状态和TERT促进器 (TERTp) 基因之间的关联.

主要方法:

在22个手术切除的TSCC组织上进行了微RNA表达概况分析,并使用nCounter Nanostring miRNA表达试验匹配了正常的桃体组织.
使用层次聚类分析来识别差异表达的miRNAs.
进行了功能性丰富分析,以确定失调的miRNAs的生物作用.

主要成果:

鉴定出TERT促进子 (TERTp) 基因是唯一与TSCC中差异表达miRNA相关的相关候选基因.
显著的miRNA表达特征与HPV阳性TSCC相关,其特征是高水平的hsa-miR-1285-5p,hsa-miR-1203,hsa-miR-663a,hsa-miR-1303,hsa-miR-33a-5p和hsa-miR-3615,以及低水平的hsa-miR-3182,hsa-miR-219a-2-3p和hsa-miR-767-3p (p = 0.009).
失调的miRNAs主要参与蛋白质结合和细胞组件功能.

结论:

特定的miRNAs,特别是hsa-miR-1285-5p和hsa-miR-663a,与HPV阳性TSCC显著相关,特别是那些具有TERTp突变的TSCC.
这些已识别的miRNAs作为早期TSCC检测的潜在生物标志物具有前途.
这些发现表明,这些miRNAs可以被探索为HPV阳性TSCC的新疗法标.

关键词:

在 TERT 促销基因基因中.在hsa-miR-1285-5pp中使用.这是一个hsa-miR-663a.这就是miRNAs.耳鼻喉 (oropharynx) 是一个状细胞癌的癌症.桃体桃体是一个

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