相关实验视频
Updated: Jul 12, 2025

11:27
Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
15.5K
在DNA中电荷逆转的值包括单环波动
Matthew D Sievert1, Marilyn F Bishop2, Tom McMullen2
1Department of Physics, New Mexico State University, Las Cruces, NM 88003-8001, USA.
Entropy (Basel, Switzerland)
|October 28, 2023
概括
聚电解质模型中的波动显著降低,即使低于非相互作用水平. 这一发现对理解溶液中的DNA电荷凝结产生影响.
科学领域:
- 生物物理学的生物物理.
- 物理化学 物理化学
- 计算生物学 计算生物学
背景情况:
- 充电溶液中的DNA表现出复杂的行为,如电荷逆转和凝结.
- 多电解质在这些观察到的现象中起着至关重要的作用.
- 了解这些相互作用需要先进的理论模型.
研究的目的:
- 在DNA上计算多电解质的和电荷分布.
- 为了研究波动的影响超出平均场理论.
- 用网格气体方法建模聚电解质吸附.
主要方法:
- 场理论方法包含一个循环的顺序波动.
- 用于双电荷的多电解质二次体的格子气体模型.
- 计算和电荷分布.
主要成果:
- 在弱结合模式下,波动条件使缩大约50%.
- 二元体和DNA之间的排斥与化学潜力竞争.
- 由于相关性而导致的缩显著降低了总.
结论:
- 波动在多电解质-DNA相互作用中起着至关重要的作用.
- 该模型提供了对由相关性驱动的变化的见解.
- 该方法可以扩展到更复杂,更现实的模型.
相关概念视频
Electrostatic Boundary Conditions
481
Consider an external electric field propagating through a homogeneous medium. When the electric field crosses the surface boundary of the medium, it undergoes a discontinuity. The electric field can be resolved into normal and tangential components. The amount by which the field changes at any boundary is given by the difference between the field components above and below the surface boundary.
The surface integral of an electric field is given by Gauss's law in integral form and is related to...
The surface integral of an electric field is given by Gauss's law in integral form and is related to...
481
DNA Topoisomerases
31.4K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
31.4K
DNA as a Genetic Template
22.0K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.0K
Entropy within the Cell
10.7K
A living cell's primary tasks of obtaining, transforming, and using energy to do work may seem simple. However, the second law of thermodynamics explains why these tasks are harder than they appear. None of the energy transfers in the universe are completely efficient. In every energy transfer, some amount of energy is lost in a form that is unusable. In most cases, this form is heat energy. Thermodynamically, heat energy is defined as the energy transferred from one system to another that...
10.7K
Energy Associated With a Charge Distribution
1.5K
The work done to bring a charge through a distance r is given by the potential difference between the initial and the final position. To assemble a collection of point charges, the total work done can be expressed in terms of the product of each pair of charges divided by their separation distance, defined with respect to a suitable origin. Solving this expression gives the energy stored in a point charge distribution.
1.5K
The DNA Helix
20.8K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
20.8K

