超声响应纳米气泡用于联合siRNA-氧化物纳米粒子向骨细胞输送
Pedram Sotoudeh Bagha1, Elayaraja Kolanthai2, Fei Wei1
1BiionixTM (Bionic Materials, Implants & Interfaces) Cluster, Department of Medicine, University of Central Florida College of Medicine, Orlando, FL 32827, USA.
Pharmaceutics
|October 28, 2023
概括
这项研究引入了一种超声波触发的纳米泡系统,用于输送Cathepsin K siRNA和氧化纳米粒子,用于治疗骨质疏松症等骨疾病. 该系统有效地减少了骨质再吸收,并增强了体外骨的形成.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 再生医学是一种再生医学.
背景情况:
- 骨质疏松症是一种骨疾病,其特点是骨质减少和骨折风险增加.
- 目前治疗骨质疏松症的方法有局限性和副作用.
- 需要新的治疗策略来有效治疗骨疾病.
研究的目的:
- 开发一种基于超声波介导的纳米泡 (NB) 的基因传递系统,用于治疗骨疾病.
- 为了共同提供Cathepsin K (CTSK) siRNA和氧化纳米粒子 (CeNPs),用于骨再生.
- 评估该系统在减少骨质再吸收和增强骨质形成方面的有效性.
主要方法:
- 使用TEM和XPS合成和表征CeNP.
- 通过超声波将被吸收的CTSK siRNA和CeNP吸收到NB上.
- 在低强度脉冲超声波 (LIPUS) 刺激后测量了siRNA和CeNP的释放概况.
- 在使用阿利沙林红色染色 (ARS) 的hMSC中评估骨质质促进.
- 在使用TRAP染色的OCP中评估骨质结晶抑制.
主要成果:
- 在LIPUS刺激后的特定度和时间点达到CTSK siRNA和CeNP的最大释放.
- 在用NB-(CTSK siRNA + CeNPs) 处理的hMSC中矿化显著增加.
- 在接受NB-(CTSK siRNA + CeNPs治疗的OCP中,骨质细胞形成的显著减少).
结论:
- 开发了一种对超声波敏感的新型NB系统,用于同时传递基因沉默siRNA和CeNP.
- 在实验室中证明了该系统在减少骨吸收和增强骨形成方面的潜力.
- 这个平台有望在未来的体内应用中治疗骨折和骨质疏松症等疾病.
相关概念视频
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
02:30Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...