对殖民化因素和机制的影响 参与由黄素介导的抗微生物声光动力学失活
Fernanda Alves1, Sebastião Pratavieira1, Natália Mayumi Inada1
1Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo CEP 13560-970, Brazil.
Pharmaceutics
|October 28, 2023
概括
声光动力学失活 (SPDI) 结合光动力学 (PDI) 和声动力学 (SDI) 失活,证明对黄金菌生物膜更有效. 所有方法都降低了细菌的新陈代谢,粘附和生物膜的形成,SPDI显示出优越的抗菌作用.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 生物医学工程 生物医学工程
背景情况:
- 光动力失活 (PDI) 和声动力失活 (SDI) 是已确立的抗菌战略.
- 声光动力学无活化 (SPDI),PDI和SDI的联合应用,显示了增强的疗效.
- 黄金葡萄球菌生物膜是一个重大挑战,原因是它们对传统治疗的耐药性.
研究的目的:
- 评估PDI,SDI和SPDI的疗效,使用黄素作为抗黄球菌生物膜的光敏剂.
- 评估这些治疗对细菌新陈代谢,粘附,生物膜形成和细胞结构的影响.
- 在不同的照射条件下测量反应性氧物种 (ROS) 生产和黄素降解.
主要方法:
- 在Staphylococcus aureus生物膜上对PDI,SDI和SPDI治疗进行比较分析.
- 细菌代谢的量化,粘附能力和生物膜生物质.
- 测量反应性氧物种 (ROS) 生产和黄素光谱变化.
- 细菌细胞和细胞外基质中的结构变化的微观分析.
主要成果:
- 与单独使用PDI和SDI相比,SPDI显示出更高的生物膜失活性.
- 所有治疗都显著减少了细菌的粘附,新陈代谢和生物膜的形成.
- 代谢活动减少了89% (PDI),82% (SDI) 和90% (SPDI).
- SPDI显示了总生物膜生物质的最大减少 (35%低于对照).
- 所有的治疗都产生了ROS,并诱导了细胞和细胞外基质的结构损伤.
- 暴露于光线导致黄素显著降解,而超声波对其光谱的影响很小.
结论:
- SPDI是针对金黄色葡萄球菌生物膜的一种高度有效的抗微生物策略,单独超过了PDI和SDI.
- PDI和SDI的结合方法在打击生物膜形成和细菌生存能力方面提供了显著的优势.
- 这些发现凸显了SPDI作为抗药性细菌感染的先进治疗选择的潜力.
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